骨形态发生蛋白在形觉剥夺性近视眼后巩膜中表达的变化  

Expression of bone morphogenetic protein in sclera of form deprivation myopic eye

作　　者：王青[1] 刘筱楠 薛美兰[3] 刘桂波[1] 王楠[1] 赵桂秋[1] 

机构地区：[1]青岛大学医学院附属医院眼科,266003 [2]青岛市立医院眼科,266000 [3]青岛大学医学院生物化学与分子生物学教研室,266000

出　　处：《中华实验眼科杂志》2013年第12期1105-1109,共5页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金青年基金项目（81300790）

摘　　要：背景眼球伸长过程伴随着巩膜的广泛重塑，而这种重塑受多种生长因子的调控。以往的研究证实转化生长因子-β（TGF-β）在近视形成和发展过程中发挥作用。骨形态发生蛋白（BMPs）属于TGF-β超家族，其在近视的发生中是否发挥作用及如何发挥作用尚不清楚。目的观察豚鼠形觉剥夺性近视（FDM）眼后巩膜中BMPs的表达变化，探讨其在近视后巩膜重塑中的作用。方法1～2周龄花色豚鼠30只，以随机数字表法随机分为实验组和正常对照组，每组15只。任意选择实验组豚鼠的一侧眼作为实验眼，应用半透明眼罩连续遮盖14d以建立FDM动物模型，另一侧眼作为对照眼。形觉剥夺前后所有动物眼经检影验光获得屈光度，用A型超声法测量眼轴长度。造模后第15天取豚鼠后巩膜组织，分别用逆转录PCR（RT—PCR）和Westernblot法检测各组豚鼠后巩膜中BMPsmRNA及其蛋白的表达。结果实验14d后，豚鼠遮盖眼的屈光度为（一0．48±0．51）D，与对侧眼的（3．22±0．34）D比较，差异有统计学意义（t=-12．814，P=0．000），与正常对照眼的（2．97±0．70）D比较，差异有统计学意义（t=-11．878，P=0．000）。豚鼠遮盖眼的眼轴长度为（8．30±0．05）mm，明显长于对侧眼的（8．11±0．06）mm和正常对照组（8．06±0．06）mm，差异均有统计学意义（t=7．230、9．084，均P=0．000）。正常豚鼠后巩膜可表达BMP-2、BMP-4、BMP-5mRNA，豚鼠遮盖眼后巩膜中BMP-2mRNA和BMP-5mRNA的相对表达值分别为0．41-±0．11和0．65±0．06，较对侧眼的0．62-±0．07和0．84±0．03分别下降了34．48％和23．67％，差异均有统计学意义（t=2．838，P=0．017；t=2．524，P=0．028）；豚鼠遮盖眼后巩膜中BMP-2和BMP-5蛋白表达的相对值分别为0．44±0．06和0．70-±0．05，较对侧眼的0．61±0．05和0．824-0．03分别下降了23．42％和15．21％，差异均有统Background It is well known that sclera remodeling occurs during axial elongation in myopia under the control of growth hormone or its downstream effectors. The role of transforming growth faetor-~ （TGF-~） in myopia has been determined in previous studies. Bone morphogenetie protein （BMP） is one of members of the TGF-[~ superfamily,hut if it plays an important role in the genesis and development of myopia is not completely clear. Objective This study was to identify the presence of BMPs in normal guinea pigs sclera and investigate the change of BMPs in the sclera in form-deprivation myopia （FDM） of guinea pigs. Methods Thirty young guinea pigs were randomized into normal control group and experimental group using table of random number. FDM models were established by occluding unilateral eyes of guinea pigs with a translucent lens for 14 days in the experimental group, and the fellow eyes served as the controls. Diopter of all eyes was tested by retinoscopy optometry, and ocular axial length was measured by A-sonography before and after modeling. Posterior sclera tissue of the animals was obtained on 14 days,and the relative expression level of BMPs mRNA and protein were assayed by reverse transcription PCR （RT-PCR） and Western blot. The use and care of the animals complied with ARVO Statement. Results On 14 days after occluding of unilateral eyes, the refraction diopter of the experimental group was （ -0.48-±0.51 ） D, and that of the fellow eyes was （3.22-±0.34）D, showing a significant difference between them （t =-12. 814,P = 0. 000）. Also,a significant difference in the diopter was seen between the experimental group and normal control group （[-0.48-±0.51]D vs. [ 2.97-±0.70] D,t = -11.878,P=0.000）. Axial length was （8.30_±0.05）mm in the experimental group, （ 8. 11 _±0.06 ） mm in the fellow eyes and （ 8.06-±0.06 ） mm in the normal control group, showing a significant increase in the experimental group compared with the fellow eyes and normal control group （t =

关 键 词：转化生长因子-Β 骨形态发生蛋白 近视 形觉剥夺性 巩膜 

分 类 号：R778.11[医药卫生—眼科]