二甲亚砜促进5-氨基酮戊酸光动力疗法对A431细胞杀伤和小鼠皮肤鳞癌的抑制效应  

Enhanced 5-Aminolevulinic acid-based photodynamic effects by Dimethyl sulfoxide on A431 cytotoxicity in vitro and inhibition of skin squamous carcinoma in mice

作　　者：倪文琼[1] 张晨阳[1] 季艳芳[1] 肖春才[1] 张雪[1] 李存涛[1] 

机构地区：[1]郑州大学附属郑州中心医院皮肤科,河南郑州450007

出　　处：《现代肿瘤医学》2013年第12期2674-2677,共4页Journal of Modern Oncology

基　　金：郑州市普通科技攻关项目(编号:112PPTSF312-1)

摘　　要：目的:研究DMSO参与5-氨基酮戊酸介导的光动力疗法(ALA-based photodynamic therapy,ALAPDT)对A431细胞杀伤和小鼠皮肤鳞癌的抑制效应,探讨治疗皮肤肿瘤的优化作用。方法:DMSO干预后,采用流式细胞仪检测A431细胞内PpⅨ水平和PDT后细胞凋亡率。激光共聚焦显微镜检测小鼠鳞癌瘤体组织的荧光强度,比较PDT后各组鳞癌小鼠的抑瘤率。结果:实验组A431细胞PpⅨ荧光强度率高于ALA组和空白对照组(P<0.01),与DMSO含量呈浓度依赖性。PDT后实验组细胞凋亡率高于ALA组和空白对照组(P<0.01)。实验组瘤体组织PpⅨ荧光强度值高于10%ALA对照组和10%DMSO对照组(P<0.01),与DMSO含量呈浓度依赖性。实验组鳞癌小鼠PDT治疗后,抑瘤率高于对照组(P<0.05),并有量效依从关系。结论:DMSO可促进ALA-PDT对A431细胞杀伤和小鼠皮肤鳞癌的抑制效应。Objective：To study the effect of enhanced 5 - Aminolevulinic acid - based photodynamic therapy （ALA -PDT） by DMSO on A431 cytotoxicity in vitro and inhibition of mice skin squamous cell carcinoma（MSCC） in vivo. Methods ： A431 cells were co - cultured with DMSO. Apoptosis after ALA - PDT and protoporphyrin Ⅸ （ Pp Ⅸ ） concentration of A431 cells co - cultured with DMSO were detected by flow cytometry. The fluorescence intensity in MSCC frozen sections was detected by laser scanning confocal microscopy. After ALA - PDT the MSCC suppressing rates were compared. Results：The PpⅨ fluorescence intensity of A431 cells in the experimental/therapeutic group showed DMSO dose dependant manner, was higher than that in the ALA alone or control group （P 〈 0.01 ）. After ALA - PDT,the A431 cell apoptotic rates in the experimental group were higher than that in the ALA alone or control group （P 〈 0.01 ）. The PpⅨ fluorescence intensity in the experimental group showed DMSO dose dependant manner was higher than that in the ALA alone or control group （ P 〈 0.01 ）. After ALA - PDT the tumor suppressing rates showed DMSO dose dependant manner in therapeutic group （ P 〈 0.05 ）. Conclusion ： DMSO can promote ALA - PDT effects on A431 cytotoxieity and MSCC inhibition.

关 键 词：二甲亚砜 5-氨基酮戊酸 光动力疗法 细胞凋亡 抑瘤率 

分 类 号：R730.5[医药卫生—肿瘤] R739.5[医药卫生—临床医学]