肝细胞癌中NDRG-1与上皮细胞钙粘蛋白的表达及调控关系  

The expression and regularive relationship of NDRG-1 and E-cadherin in hepatocellular carcinoma

作　　者：赵丹懿[1] 吴涛[1] 宋磊[1] 蒋葵[1] 杨晓燕[1] 张弦[1] 

机构地区：[1]大连医科大学附属第二医院肿瘤内科,辽宁大连116027

出　　处：《现代肿瘤医学》2013年第12期2691-2693,共3页Journal of Modern Oncology

摘　　要：目的:研究NDRG-1与上皮细胞钙粘蛋白(E-cadherin)在肝细胞癌组织中的表达和相关性,明确在肝细胞癌细胞中二者之间的调控关系。方法:实时定量PCR检测原发无转移肝细胞癌和原发伴转移肝细胞癌组织中NDRG-1和E-cadherin的表达。真核表达载体pcDNA3.3-NDRG1转染肝细胞癌HepG2细胞,Western blot检测NDRG-1和E-cadherin蛋白的表达。结果:与原发无转移肝细胞癌相比,原发伴转移肝细胞癌组织中的NDRG-1表达呈现显著上调,E-cadherin表达呈现显著下调,且二者呈显著负相关。转染后,NDRG-1蛋白的表达显著上调,而E-cadherin蛋白的表达显著下调。结论:NDRG-1和E-cadherin与肝细胞癌的转移相关,NDRG-1在肝细胞癌细胞中能够下调E-cadherin的表达。Objective： To research the expression and relationship of NDRG - 1 and E - cadherin in hepatocellular carcinoma, and the regulation relationship between them in hepatocellular carcinoma cells. Methods ： Real - time PCR was used to detect NDRG - 1 and E - cadherin mRNA in primary hepatocellular carcinoma without metastasis and pri- mary hepatocellular carcinoma with metastasis. After the expression vector pcDNA3.3 - NDRG1 was transfected to HepG2 cell, the expression of NDRG - 1 and E - cadherin protein was deteced by Western blot. Results ： The mRNA expression of NDRG - 1 in primary hepatocellular carcinoma with metastasis was much higher than that in primary hepatocellular carcinoma without metastasis, but the expression of E - cadherin was much lower, and there was a posi- tive relationship between them. After transfection, the expression of NDRG - 1 protein increased significantly, and the expression of E - cadherin decreased significantly. Conclusion ： The NDRG - 1 and E - cadherin gene have a relation- ship with metastasis of hepatoeellular carcinoma, NDRG - 1 can down - regulate the expression of E - eadherin in hepatocellular carcinoma cells.

关 键 词：肝细胞癌 转移 NDRG-1 上皮细胞钙粘蛋白 

分 类 号：R735.7[医药卫生—肿瘤]