四种乳腺癌相关基因联合检测对乳腺癌诊断的临床应用研究  

Clinical application of CK19,Ccnd1,Bcl-2 and MYC gene expression in the diagnosis of breast cancer by combined detection

作　　者：孙昌瑞[1,2] 冯林 邓君[2] 黄文芳[2] 袁红[2] 饶绍琴[2] 传良敏[2] 洪华[2] 江咏梅[1] 

机构地区：[1]四川大学华西第二医院检验科,四川成都610041 [2]四川省人民医院(四川省医学科学院) 检验科,四川成都610072 [3]四川省妇幼保健医院检验科,四川成都610000

出　　处：《现代肿瘤医学》2013年第12期2726-2728,共3页Journal of Modern Oncology

基　　金：四川省杰出青年基金资助项目(编号:2007-5-345)

摘　　要：目的:探讨核内原癌基因(nuclear oncogene,MYC)、B细胞淋巴瘤/白血病-2基因(B cell lymphoma/leukemia 2 gene,Bcl-2)、细胞周期蛋白D1(Ccnd1)和细胞角蛋白19(cytokeratin 19,CK19)基因表达水平在乳腺癌诊断和治疗监测中的应用。方法:建立荧光定量聚合酶链反应(FQ-PCR)法,并以GAPDH为内对照测定34名健康女性体检者、55例良性乳腺疾病患者和91例乳腺癌患者外周血中MYC,Ccnd1,Bcl-2和CK19的表达量。结果:MYC,Ccnd1,Bcl-2和CK19表达水平与GAPDH的比值在乳腺癌组显著高于健康女性体检者和良性乳腺疾病患者(P<0.05)。它们在正常对照组和良性乳腺疾病组间差异无统计学意义(P>0.05)。四组GAPDH差异无统计学意义(P>0.05)。MYC,Ccnd1,Bcl-2与CK19联合检测的灵敏度高于单个基因的检测。结论:FQ-PCR技术可以快速定量检测MYC,Ccnd1,Bcl-2和CK19 mRNA,四者联合检测可有效提高对乳腺癌诊断的灵敏度。Objective：To evaluate the clinical application of MYC, Ccndl, Bcl -2 and CK19 gene in the diagnosis of breast cancer by combined detection. Methods： MYC, Ccndl, Bcl -2 and CK19 of 34 health women,55 patients with benign breast disease and 91 patients with breast cancer were detected by FQ - PCR. GAPDH was used as inter- nal control. Results：MYC, Ccndl, Bcl -2 and CK19/GAPDH in breast cancer group were higher than those in health women and patients with benign breast disease groups （P 〈 0.05 ）. There was no significant difference in MYC, breast cancer, Bel- 2 and CK19/GAPDH between normal controls and benign breast disease group（ P 〉 0.05 ）. There was no difference in GAPDH among four groups（P 〉0.05）. The sensitivity of MYC,Ccndl ,Bcl-2 and CK19 gene ex- press by combined detection was higher than that of single gene detection. Conclusion： FQ - PCR is a rapid method for quantitating MYC ,Ccndl ,Bcl -2 and CK19. The combined detection can improve the sensitivity of diagnosis in breast cancer.

关 键 词：荧光定量聚合酶链反应 CK19 CCND1 Bcl-2 MYC 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]