绞股蓝茎尖组织培养和植株再生研究  

Shoot Tips Culture and Plants Regeneration in Gynostemma pentaphyllum(Thunb.)Makino in vitro

作　　者：刘松青 武成荣[2] 

机构地区：[1]四川省喜德米市中学生物工程技术研究所,616754 [2]四川师范大学化学与生命科学学院,成都610066

出　　处：《中国野生植物资源》2000年第6期60-61,共2页Chinese Wild Plant Resources

摘　　要：以绞股蓝茎尖作为外植体 ,接种于MS +1mg/LBA +0 .1mg/LNAA的培养基中 ,茎尖逐渐形成芽苗 ,芽苗分离后接种于MS +1mg/LBA +0 .1mg/LNAA的培养基中进行增殖培养 ,同时从增殖的芽苗上不断地诱导出幼苗 ,将幼苗分离转入不含激素的MS培养基中 ,幼苗生根 ,形成完整的植株。Shoot tips in Gynostemma pentaphyllum(Thunb.)Makino cultured on Murashige and Skoog basal salts medium supplemented with 1mg/L BA and 0.1mg/L NAA and buds from Shoot tips was observed.Bud seedlings were transferred to MS medium supplemented with 1mg/L BA and 0.1mg/L NAA and those were produced new seedlings.When the rootless seedlings transfered on MS medium supplemented not exogenous hormones plantlets with roots would be gotten.

关 键 词：绞股蓝 茎尖 组织培养 植株再生 

分 类 号：S567.237[农业科学—中草药栽培]