桉木自催化水解提取聚木糖研究  

Study on Autocatalytic Hydrolysis Extraction of Xylan from Eucalyptus

作　　者：房桂干[1,2,3,4] 施英乔[1,2,3,4] 丛高鹏[1,2,3,4] 丁来保[1,2,3,4] 盘爱享 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]江苏省生物质能源与材料重点实验室,江苏南京210042

出　　处：《桉树科技》2013年第3期1-7,共7页Eucalypt Science & Technology

基　　金：林业公益性行业科研专项"桉树生态经营及产业升级关键技术研究"(201104003-06)

摘　　要：采用Design-Expert软件进行响应面试验（RSM）设计,利用外循环蒸煮锅对桉木片半纤维素自催化水解制取还原糖方法进行了初步探索。结果表明反应温度、保温时间交互作用对还原糖制取量影响显著,当水解因子P〈2 500时,响应面模型/水解P因子模型对于还原糖提取率R的影响一致,即R（153℃,90 min）≈R（162℃,30 min）,R（158℃,90 min）≈R（168℃,30 min）,R（164℃,90 min）≈R（176℃,30 min）。P因子在1 000~1 500范围内水解液中低聚木糖及木糖含量最高,总浓度为9.7 g·L-1,在反应温度170℃、保温40 min（P因子约1 000）条件下达到最优水平。当P因子〉2 000时糖组分总量呈现下降趋势,通过FT-IR、GC-MS分析,单糖组分大量转化为糠醛等其他降解产物。This response surface methodology experiment was designed to include the three easy-to-control independent variables the liquid-solid ratio, the highest temperature and the holding time, based on the Box-Behnken experimental design principles using Design-Expert software. When hydrolysis factor（P） was kept below 2 500, the effect under low temperatures with long holding times were approximately equal to that under high temperatures with short holding times for reducing sugar extraction rate（R）： R（153oC, 90 min） ≈ R（162oC, 30 min）, R（158oC, 90 min） ≈ R（168oC, 30 min）, R（164oC, 90 min） ≈ R（176oC, 30 min）. With P-factor in this range, a linear relationship was found between this factor P and the response value R（reducing sugar/oven-dry wood, g·g-1）. It was noteworthy that there was more xylo-oligosaccharide than xylose with the P-factor ranging from about 1000 to 1 500, and it was also observed that an extraction temperature of 170oC provided better conversion of valuable xylo-oligosaccharide（about 55%） and xylose in wood-based bio-refinery system, especially under the condition of auto-hydrolysis for 40 minutes and P-factor about 1000. Combined utilization of fourier transformed infrared spectrometry（FT-IR） and gas chromatography–mass spectrometry（GC-MS） proved useful for establishing detailed mass balances of the auto-hydrolysis process. Analyses of results obtained showed that with the increase of auto-hydrolysis intensity, low degree polymerized xylan can be further converted into small quantities of furfural and other degradation products.

关 键 词：桉木 自催化水解 低聚木糖 生物质精炼 

分 类 号：S781[农业科学—木材科学与技术]