酒精性肝纤维化及肝癌大鼠Klf6及基因的表达  

The Effects of Different Liquor on the Expression of Klf 6 and Related Gene in Liver of Rats with Hepatic Fibrosis and Hepatoma

作　　者：孙江龄 何常[1,2] 刘俊玲[1] 程明亮[3] 

机构地区：[1]贵阳医学院病理学教研室,贵州贵阳550004 [2]贵阳医学院附院病理科,贵州贵阳550004 [3]贵阳医学院附院感染科,贵州贵阳550004

出　　处：《贵阳医学院学报》2013年第6期588-592,597,共6页Journal of Guiyang Medical College

基　　金：国家重点研究计划(973)课题(NO.2011CB512114);贵州省科技计划课题(黔科合SZ[2011]3001)

摘　　要：目的:研究不同白酒对酒精性肝纤维化、肝硬化合并肝癌大鼠肝组织中Kruppule样因子6(Klf 6)及相关基因表达的影响。方法:运用2种白酒和黄曲霉素B1(AFB1)对大鼠进行处理,制备酒精性肝纤维化、肝硬化合并肝癌大鼠模型。用免疫组织化学法检测模型大鼠肝脏组织Klf 6、转化生长因子-β1(TGF-β1)、p21及α-平滑肌动蛋白(α-SMA)的表达水平。RT-PCR技术检测大鼠肝脏组织中Klf 6基因mRNA表达水平。结果:成功制备了酒精性肝纤维化、肝硬化合并肝癌大鼠模型,不同白酒诱导大鼠肝纤维化、肝硬化合并肝癌的程度不同。Klf 6、p21在白酒A组(AD组和AG组)的表达率高于白酒B组(BD组和BG组),差异有统计学意义(P<0.05);TGF-β1,α-SMA在白酒B组(BD组和BG组)表达高于白酒B组(BD组和BG组),差异有统计学意义(P<0.05)。RT-PCR结果显示,白酒A组(AD组和AG组)肝组织Klf 6基因的mRNA表达高于白酒B组(BD组和BG组),差异有统计学意义(P<0.05)。结论:不同白酒对酒精性肝纤维化,肝硬化合并肝癌大鼠肝脏Klf 6、p21、TGF-β1和α-SMA的表达不同,可能是导致酒精性肝病(ALD)的程度不同的机制之一。Objective： To discuss the expression of Klf 6 and related gene in hepatic fibrosis, and hepatocirrhosis with hepatoma tissue rats administered with different liquor. Methods： The hepatic fi- brosis and hepatocirrhosis with hepatoma rat model was made with aflatoxin B1 （ AFB1 ） and 2 liquor （liquor A and B with low and high content） respectively, and the control rats were administered with distill water. The protein expressions of Kruppel-like factor 6 （Klf 6）, transforming growth factor-J51 （TGF-[M）, p21, and alpha smooth muscle actin （ot-SMA） in liver were detected with immunohisto- chemistry method, and the mRNA level were evaluated with RT-PCR after the rats were decapitated. Results： The hepatic fibrosis and hepatocirrhosis with hepatoma rat model was made successfully, and rat models induced by different liquor showed pathological changes with different severity. The expres- sion of Klf 6 （ protein and mRNA level） AD） and high-content group （group AG BD） and high-content group （group BG and p21 （protein level） of liquor A low-content group （group were higher than those of liquor B low-content group （ group with statistical significance （P 〈 0.05 ）. However, the pro-tein expression of TGF-I31 and oL-SMA increased in BD and BG groups contrasted with AD, AG and control groups （ P 〈 0.05 ）. Conclusions： The degree of the effects of different liquor on the expression of Klf 6, TGF-B1, p21, and ot-SMA is different, which may contribute to the different inducing effects on hepatic fibrosis, hepatocirrhosis and hepatocirrhosis with hepatoma.

关 键 词：肝疾病 酒精性 纤维化 肝硬化 酒精性 肝肿瘤 Kruppule样因子6 蛋白质 P21 α-平滑肌动蛋 白 

分 类 号：R575[医药卫生—消化系统] R735.5[医药卫生—内科学]