功能化纳米氧化石墨烯微粒的制备及对脑胶质瘤U251细胞的靶向显像作用  

Preparation of functionalized nano-graphene oxide particles and their target imaging role in glioma U251 cells

作　　者：邓跃飞[1] 李忠军[1] 庞家栋 张黎明[2] 

机构地区：[1]中山大学附属孙逸仙纪念医院神经外科,广州510120 [2]中山大学高分子化学研究所,广州510275

出　　处：《中华神经医学杂志》2013年第12期1234-1238,共5页Chinese Journal of Neuromedicine

基　　金：广东省自然科学基金（10151008901000029）;广东省科技计划（20118031800038）

摘　　要：目的制备功能化纳米氧化石墨烯（nano-GO-Tf-FITC）微粒，并研究其在近红外线（NIR）照射下对脑胶质瘤U251细胞的靶向荧光显像作用。方法将氧化石墨烯（GO）超声振荡制得纳米单层GO（nano—GO）微粒，以聚赖氨酸叠氮基团（poly-L—lysine—G3）连接靶向分子转铁蛋白（Tf）和荧光分子异硫氰酸荧光素（FITC），制备出功能化nano．GO—Tf-FITC微粒，应用透射电镜在一定倍数下观察并拍摄样品形貌，测定样品粒径。将培养的脑胶质瘤U251细胞分成3组，即靶向实验组（加入nano-GO—Tf-FITC微粒孵育）、平行对照组（加入抗CD71．FITC试剂孵育）和非靶向对照组（加入nano．GO．FITC微粒孵育），采用荧光显微镜检测其荧光显像。结果在高分辨率透射电镜下nano—G0为20～100nm单片层纳米颗粒，功能化nano—GO．TgFIT微粒为分散在〈100nm的单片层。在荧光显微镜下，靶向实验组和平行对照组U251细胞均呈黄绿色，非靶向对照组U251细胞则无显像。结论成功制备了功能化nano．GO．Tf-FITC微粒，并且其对脑胶质瘤U251细胞具有明显靶向显像作用。Objective To prepare the fimctionalized nano-graphene oxide （nano-GO） particles, and study their targeted fluorescence imaging effects on glioma U251 cells under near infrared （NIR） irradiation. Methods Single-layer nano-GO particles were obtained after ultrasonic oscillation, and then connected with transferrin （Tf） and fluorescein isothiocyanate （FITC） through poly-L-lysine-G3 to prepare functionalized nano-GO-Tf-FITC particles. Sample morphology was observed and the particle sizes were measured under certain transmission electron microscope （TEM）. Glioma U251 cells were employed and divided into targeted experimental group （adding functionalized nano-GO-Tf-FITC particles）, parallel control group （adding anti-CD71-FITC reagent） and non-targeted experimental group （adding nano-GO-FITC particles）. Fluorescence imaging of these groups was determined by fluorescence microscopy. Results Single-layer particles with a range of 20-100 nm were observed under high TEM, and the functionalized nano-GO-Tf-FITC particles were distributed in a pattern of single layer with a diameter smaller than 100 nm. Yellow green color was monitored in the targeted experimental group and parallel control group, and no imaging was monitored in the non-targeted experimental group because of no transferring integration. Conclusion Functionalized nano-GO-Tf-FITC particles are successfully prepared, and show marked targeted imaging effects on glioma U251 cells.

关 键 词：神经胶质瘤 氧化石墨烯 转铁蛋白 纳米化 荧光显像 

分 类 号：R739.4[医药卫生—肿瘤]