纳秒级陡脉冲电场治疗裸鼠恶性黑色素瘤的疗效观察  

The Anti-tumor Efficacy of Nanosecond Pulsed Electric Fields on the Mouse with Melanoma Xenograft in Vivo

作　　者：彭巧[1] 董守龙[2] 郭飞[2] 姚陈果[2] 唐均英[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016 [2]重庆大学输配电装备及系统安全与新技术国家重点实验室,重庆400030

出　　处：《生物医学工程学杂志》2013年第6期1302-1308,共7页Journal of Biomedical Engineering

基　　金：国家自然科学基金重点项目资助(50637020);教育部博士点基金项目资助(20050611020);重庆市科委科技项目资助(2005AA5008-3)

摘　　要：为观察纳秒级陡脉冲电场(nsPEFs)治疗绿色荧光蛋白标记的恶性黑色素瘤细胞裸鼠皮下瘤的疗效,本实验采用活体荧光成像技术检测治疗前以及治疗后48h、10d的皮下瘤荧光变化,组织学检测治疗后90d治疗局部效果。数码相机随访治疗后疤痕变化。结果显示,治疗组皮下瘤荧光强度及面积于治疗后48h大部分减少,至治疗后10d完全消失,对照组则逐渐增加。组织学证实治疗组治疗后90d治疗原位未见肿瘤细胞。皮下瘤治疗后治疗区域出现结痂,随时间推移,疤痕逐渐变浅。结果表明:nsPEFs可以有效治疗裸鼠恶性黑色素瘤,且疤痕少,操作简单,它可能成为浅表肿瘤临床治疗的一种新途径。This study was conducted to investigate the anti tumor efficacy of nanosecond pulsed electric fields （nsPEFs） on the mouse with A375-GFP melanoma xenograft in vivo. In vivo fluorescence image analysis system was used in this study to evaluate the effects of nsPEFs on human melanoma A375 cell xenograft. On the Day 90 af- ter pulse delivery, the skin that had contained A375 cell xenograft was surgically excised and pathologically evalua ted. The changes of scar were recorded by digital camera. The experiment revealed that significant changes in fluo rescence value trend and amplitude were found in the treated group from those in the control group. The fluorescence of tumor in the treated group decreased mostly 48 h after the treatment and completely disappeared 10 d after the treatment, while that in control group was increased gradually. Surgical excision of the area confirmed a complete pathologic response. Within a few days after the nsPEFs treatment, a hard scab formed at the treatment region. The scab fell off by the end of the second week. As time went on, the scar gradually became faded and all xenograft tumors were disappeared without recurrence. From the experiment, we learn that nsPEFs can bring good therapeutic effect. It may provide a new approach for the clinical treatment of superficial tumors.

关 键 词：纳秒级陡脉冲电场 有效性 活体荧光成像 绿色荧光蛋白 人黑色素瘤 

分 类 号：R739.5[医药卫生—肿瘤]