反应性胶质细胞增生、神经炎症与脑积水关系的实验研究  

Experimental research of reactive gliosis and nearoinflammation in rats with hydrocephalus

作　　者：徐昊[1] 王占祥[2] 张绍林[2] 谭国伟[2] 朱宏伟[2] 黄才权[2] 张飞飞[2] 刘峰[2] 

机构地区：[1]安徽省立医院神经外科,合肥230001 [2]厦门大学附属第一医院神经外科

出　　处：《中华小儿外科杂志》2013年第12期927-932,共6页Chinese Journal of Pediatric Surgery

基　　金：基金项目：国家自然科学基金（81271332）

摘　　要：目的探究反应性胶质细胞增生、神经炎症与大鼠脑积水的关系。方法选取成年雄性SD大鼠35只，采用随机数字表法分为脑积水模型组（25只）、假手术组（5只）、正常假手术组（5只）。采用高岭土脑室内注射法建立脑积水模型，术后3周进行磁共振检查，抽取脑脊液进行白细胞介素-18（IL-18）的酶联免疫吸附测定（ELISA）检测，并对脑组织的神经胶原纤维酸性蛋白（glialfi—brillaryacidicprotein，GFAP）、同种异体移植炎症因子-1（allograftinflammatoryfactor-1，AIF-1）进行实时定量聚合酶链反应（RT-PCR）、蛋白免疫印迹（wB）和免疫组织化学检测，结果行统计学分析。结果造模组均有脑积水形成，与正常组和假手术组相比，造模组大鼠侧脑室体积明显增大（P〈0．05）。GFAP、AIF1、IL-18表达量在脑积水大鼠中均升高，且差异有统计学意义（P〈0．05）。相关性分析显示GFAP、IL18与脑积水严重程度呈正相关。结论反应性胶质细胞增生和神经炎症与脑积水严重程度呈正相关，有潜力成为脑积水发生发展的评价指标。能否通过抗感染和抑制胶质增生治疗脑积水，成为今后研究的新方向。Objective To investigate the relationship between reactive gliosis and hydrocephalus at both cellular and molecular levels. Methods Thirty-five SD rats were randomly divided into normal group （n = 5）, sham operation group （n = 5） and model group （n = 25）. Hydrocephalic rat models were induced by intraventricular injections of 3% kaolin and the ventricular dilatation were examined by MRI 3 weeks postoperatively. The model group were subdivided into the mild group （n = 5）, mod- erate group （n = 7） and sever group （n = 9） according to the degree of ventricular dilatation. Interleu- kin- 1 8 （IL- 1 8）, glial fibrillary acidic protein （GFAP）, Iba-1 （ ionized calcium-binding adaptor molecule） were detected by ELISA and RT-PCR. Immunohistochemistry, Western blot and correlation analysis were conducted. Results Compared to the normal group and the sham operation group, the ventricle of the model group was obviously enlarged（P〈0. 05）. The expression of GFAP and Iba-I were in- creased（P〈0.05） in brain tissue in the model group and IL-18 was also increased in cerebral spinal fluid （CSF） sample. Correlation analysis revealed that the increase was positively correlated with the severity of ventricular dilatation. Conclusions These results indicate that gliosis and inflammation in- crease dramatically in experimental hydrocephalus and can be regarded as potential parameters for eval- uating hydrocephalus.

关 键 词：脑积水 大鼠 神经胶质增生 炎症 

分 类 号：R742.7[医药卫生—神经病学与精神病学]