ANXA2对人结直肠癌caco2细胞行为及细胞骨架结构的调节  

ANXA2 Regulates the Behaviors and Cytoskeletal Structure of caco2 Cells in Human Colorectal Cancer

作　　者：张彩霞[1] 武浩杰[1] 奉艳 王晓静[1] 胡妍妍[1] 贾良杰 侯颖春[1] 

机构地区：[1]陕西师范大学肿瘤分子细胞生物学实验室,西安710062

出　　处：《中国细胞生物学学报》2013年第12期1741-1747,共7页Chinese Journal of Cell Biology

摘　　要：ANXA2是Annexin蛋白家族中的重要成员,研究证实,其在多种肿瘤组织中异常表达,且在细胞功能及命运的调节中扮演重要角色。为了准确研究ANXA2对细胞行为及细胞骨架结构的调节以及该调节对肿瘤发展过程中的影响,该实验室构建了敲除ANXA2基因的人结直肠癌细胞系(ANXA2–/–caco2),研究ANXA2对细胞的生物学行为及结构的调节。结果显示,ANXA2表达的祛除明显抑制caco2细胞的增殖和迁移能力(P<0.05),但对其凋亡没有显著影响;敲除ANXA2显著降低F-actin的表达,且抑制caco2细胞伪足和微绒毛的发育,这也进一步验证了ANXA2的敲除影响caco2细胞的增殖与迁移能力。该研究结果在靶基因敲除的条件下从更加客观的形态学角度进一步支持了ANXA2对caco2细胞癌发展有关特性的重要调节作用,以及其作为癌基因治疗靶基因的重要潜在性。Annexin A2 （ANXA2） is an important member of Annexin family and it was confirmed to be abnormally expressed in various cancers and has important roles in cellular functions and fate. To further study the specific effects of ANXA2 on the behaviors and cytoskeletal structure exactly, the research was conducted to construct a ANXA2-/-caco2 cell line and to study the roles of ANXA2 playing in the biological behavior and cytoskeletal structure for caco2 cells. The results showed that the knockout of ANXA2 could significantly inhibit proliferation and migration in ANXA2/-caco2 （P〈0.05）, but it had no clear change on apoptosis. Depletion of ANXA2 in caco2 cells remarkably decreased the expression of F-actin and inhibited the development of pseudopodium and microvilli, as evidenced by decreased proliferation and migration. Our data from the perspective of morphology objectively support that ANXA2 plays significant part in regulating the development of caco2 cells under the condition of target gene knockout, and the data also shows that ANXA2 may be a potential target for therapeutic strategies.

关 键 词：膜联蛋白A2 人结直肠癌 细胞增殖 细胞迁移 细胞凋亡 肌动蛋白 伪足 

分 类 号：R735.3[医药卫生—肿瘤]