肺癌患者外周血中单个核细胞表面程序性死亡蛋白1及其受体的表达和生物学意义  

Expression of PD-1/PD-L1 in peripheral blood mononuclear cells in lung cancer patients and its biological significance

作　　者：胥萍[1] 陈慧[1] 陈永井[2] 陈延斌[3] 顾国浩[4] 吴妹英[1] 吴敏娟[1] 王雪峰[2] 张学光[2] 

机构地区：[1]苏州市第五人民医院检验中心苏州市感染免疫重点实验室,215007 [2]苏州大学医学生物技术研究所 [3]苏州大学附属第一人民医院呼吸科 [4]苏州大学附属第一人民医院检验科

出　　处：《中华肿瘤杂志》2013年第12期910-913,共4页Chinese Journal of Oncology

基　　金：苏州市科学技术局资助项目（SYS201155、SS201246）;江苏省卫生厅资助项目（KF200974）

摘　　要：目的分析肺癌患者外周血中单个核细胞表面协同刺激分子程序性死亡蛋白1（PD-1）及其受体（PD-L1）的表达特性，并探讨其生物学意义。方法取133例肺癌患者、25例肺部感染患者和23例健康志愿者的抗凝全血100μl，采用多色免疫荧光标记和流式细胞术对外周血中单个核细胞表面PD-1和PD-L1的表达水平进行检测和分析。结果肺癌患者外周血中CD3＋CD8＋T细胞亚群的比例为（38．83±1．74）％，明显低于健康志愿者[（43．25±3．35）％，P〈0．05]。肺癌患者外周血中CD8＋CD28＋T细胞亚群的比例为（17．73±1．21）％，明显低于健康志愿者[（27．96±2．72）％，P〈0．01]。肺癌患者外周血中CD8＋CD28-T细胞亚群的比例为（21．19±1．92）％，明显高于健康志愿者[（15．18±2．93）％，P〈0．05]。肺癌患者外周血中CD8＋CD28＋T细胞表面PD-1的表达水平为（10．67±1．12）％，较健康志愿者[（5．32±1．58）％]明显增高（P〈0．01）。肺癌患者外周血中CD8＋CD28-T细胞表面PD-1的表达水平为（7．464-1．25）％，明显高于健康志愿者[（2．68±1．07）％，P〈0．01]。肺癌患者外周血中CD68＋细胞表面PD—L1的表达水平为（16．03±2．06）％，较健康志愿者[（9．72±2．00）％]明显增高（P〈0．05）。结论PD-1和PD—LI在肺癌患者外周血中的表达上调对淋巴细胞产生负性调节作用，抑制了机体免疫系统对肿瘤细胞的杀伤效应，促进了肿瘤的发展并产生免疫逃逸。Objective To analyze the expression of co-stimulatory molecules PD-1/PD-L1 in peripheral blood mononuclear cells in lung cancer patients, and to explore its biological significance. Methods One hundred and thirty-three lung cancer patients, 25 lung infection patients and 23 healthy donors were enrolled in this study. 100μl of whole blood from these subjects were collected. Multi-color immunofluorescence staining and flow cytometry were used to detect PD-1/PD-L1 expression. The results were statistically analyzed. Results The expression level of CD3＋ CD8＋ T ceils in the lung cancer patients was （ 38.83 ± 1.74 ） %, significantly lower than that in the control group [ （43.25 ± 3.35 ） %, P 〈 0.05 ]. CD8＋ CD28 ＋ T cell subset in the peripheral blood of lung cancer patients was （17. 73 ±1. 21 ）% significantly lower than that of the healthy donors [ （27.96±2.72） %, P 〈0.01 ]. The CD8＋ CD28 - T cell subset was （21. 19± 1.92）% in the lung cancer patients, significantly higher than that of the healthy control group [ （ 15.18 ± 2.93 ） %, P 〈 0.05 ]. The expression level of PD-1 on the surface of CD8＋ CD28＋ T cells was （ 10.67 ± 1.12） % in the group of lung cancer patients, significantly higher than that of the control group [ （ 5.32 ±1. 58 ） %, P 〈 0.01 ]. It was also found that the expression of PD-1 on CD8＋ CD28 - T cells was up-regnlated in the group of lung cancer patients （7.46 ±1.25 ） %, significantly higher than that of the healthy control group [ （ 2.68 ±1.07 ） %, P 〈 0.01 ]. The expression level of PD-L1 on CD68 ＋ ceils in the lung cancer patients was （ 16.03 ±2.06） %, significantly higher than that of the healthy control group [ （9.32 ± 2.00） %, P 〈 0.05 ]. Condusion Up-regulation of PD-I/PD-L1 on peripheral blood cells in lung cancer patients negatively regulates the lymphocytes, inhibits the immune response for killing tumor cells, and promotes tumor development and immune escape.

关 键 词：肺肿瘤 程序性死亡蛋白1 程序性死亡蛋白1受体 外周血 单个核细胞 

分 类 号：R734.2[医药卫生—肿瘤]