可见光诱导广西巴马小型猪视网膜变性的实验研究  

Visible light-induced retinal degeneration in Guangxi Bama miniature pigs

作　　者：刘俊[1] 徐海伟[1] 李世迎[1] 孙成[1] 黎其友[1] 陈泽华[1] 阴正勤[1] 

机构地区：[1]第三军医大学西南医院全军眼科中心,视觉损伤与再生修复重庆市重点实验室,重庆400038

出　　处：《第三军医大学学报》2013年第24期2614-2618,共5页Journal of Third Military Medical University

基　　金：国家重点基础研究发展计划(973计划;2013CB967002);国家自然科学基金(31271051;81200710)~~

摘　　要：目的采用可见光持续间断光照建立广西巴马猪的慢性视网膜变性模型,并探索活体鉴定广西巴马猪视网膜功能的方法。方法白色可见光持续间断照射3~4月龄广西巴马小型猪3~6个月,建立小型猪视网膜变性模型。通过对模型猪进行眼底照相、光学相干断层扫描(OCT)、全视野视网膜电图(fERG)和多焦视网膜电图(mfERG)等检测,从形态和功能对光诱导视网膜变性模型进行活体鉴定。结果与光照前相比,光变性巴马小型猪眼底照相无显著可见改变;OCT可见光变性猪视网膜ILM^RPE层厚度显著变薄(P<0.05);fERG可见光变性猪在暗适应时最大混合反应的a、b波和ops波振幅均有显著下降(P<0.05),在明适应时b波及P1波波幅也有显著下降(P<0.05);mfERG可见光变性猪P1波平均振幅密度显著降低(P<0.05)。结论可见光持续间断光照可诱导广西巴马小型猪视网膜出现结构和功能改变,可作为慢性视网膜变性模型。Objective To establish a chronic retinal degeneration model with Guangxi Bama miniature pig by exposing to continuous intermittent visible light, and to explore the method for examining retinal function in live Guangxi Bama miniature pigs. Methods Guangxi Bama miniature pigs aged 3 -4 months were exposed to continuous intermittent white light to induce chronic retinal degeneration. Fundus photography, optical cohe- rence tomography （ OCT）, full-field electroretinography （fERG） and multifocal electroretinography （mfERG） were used to compare the differences of retinal functions between the normal pigs and light-induced retinal de- generation pigs and to identify optical retinal degeneration in vivo. Results As compared to the normal pigs, no significant visible fundus photographic change was found in the light-induced retinal degeneration pigs. Nev- ertheless, the thickness of retinal internal limiting membrane （ILM） to retinal pigment epithelium （RPE） layer was reduced obviously in OCT （ P 〈 0.05 ）. Amplitudes of dark-adapted combined rod-cone response＇ s a waves, b waves, and ops waves and light-adapted b waves and P1 waves of fERG also decreased clearly （P 〈 0. 05 ）. Average amplitude density of P1 waves of mfERG significantly lowered （ P 〈 0.05 ）. Conclusion Continuous intermittent visible light exposure leads to producing an appropriate chronic retinal degeneration changes in the structure and function in porcine retinas, model.

关 键 词：小型猪 视网膜变性 光学相干断层扫描 电生理 

分 类 号：R-332[医药卫生] R774.13