5-氮杂-2’-脱氧胞苷联合低浓度顺铂对肺癌细胞增殖影响的实验研究  

Effects of 5-Aza-CdR and low concentration of cisplatin on non-small cell lung cancer cells

作　　者：吴俚蓉[1] 黄蕾[1] 王洁[1] 陈薇[1] 王婷婷[1] 刘雅恬[1] 王丽君[1] 宋丹[1] 

机构地区：[1]江苏省肿瘤医院放疗科,南京210009

出　　处：《临床肿瘤学杂志》2013年第11期966-969,共4页Chinese Clinical Oncology

摘　　要：目的探讨5-氮杂-2’-脱氧胞苷(5-Aza-CdR)与低浓度顺铂(DDP)联合用药对非小细胞肺癌(NSCLC)A549细胞增殖的影响。方法选择1μmol/L DDP、10μmol/L 5-Aza-CdR及两者联合分别处理体外培养的A549细胞,MTT法检测处理前后的细胞增殖活性,流式细胞仪、Hoechst 33258染色分别观察处理前后的细胞周期、凋亡率及凋亡形态学变化。结果MTT法显示,与单独1μmol/L DDP组比较,联合组能够明显抑制A549细胞的增殖,A549细胞的凋亡率亦明显增加;流式细胞仪显示,联合组作用于细胞G0/G1期,使A549细胞生长阻滞在此期,细胞的增殖指数降低;与单独1μmol/L DDP组比较,联合组A549细胞的凋亡形态学改变更明显。结论 5-Aza-CdR联合低浓度DDP能够明显抑制NSCLC A549细胞的增殖和诱导A549细胞的凋亡。Objective To investigate the combination therapy of5-aza-2＇-deoxycytidine（5-Aza-CdR） and low concentrations of cisplatin（DDP） on non-small cell lung cancer A549 cells.Methods A549 cell was treated by 1 μmol/L DDP,10μmol/L 5-AzaCdR and combination of two drugs.MTT test was used to determine the change of cell proliferation.The cell cycle and apoptosis index were analyzed by flow cytometry.The morphology of cell apoptosis was analyzed by Hoechst 33258 staining.Results 1 μmol/L DDP combined with 10μmol/L 5-Aza-CdR inhibited A.549 cells proliferation more prominently than 1 μmoL/L DDP.The apoptisis rate of A549 treated by 5-Aza-CdR and 1 μmol/L DDP was higher than that treated by 1 μmol/L DDP.1 μmol/L DDP combined with 10μmol/ L 5-Aza-CdR blocked cell cycles at G1/G0 phase and cells proliferation index reduced.The morphology of A549 cells apoptosis were changed more significantly in combined group than in 1 μmol/L DDP group.Conclusion The apoptosis rate of non-small cell lung cancer A549 cells could be increased by combination of 5-Aza-CdR and low concentrations of DDP.

关 键 词：5-氮杂-2'-脱氧胞苷 顺铂 非小细胞肺癌 A549细胞株 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]