前列腺癌LNCap/PC-3细胞模型中肿瘤转移相关基因表达的芯片分析  

Microarray analysis of tumor metastasis-related genes in LNCaP/PC-3 prostate cancer progression model

作　　者：曹开源[1,2,3,4] 徐霖[2,3,4] 王铸[2] 何霞[2] 关琳琳[2] 庹玖玲 汪杨[2,3,4] 罗燕芬[2,3,4] 钟慧玲[2,3,4] 沈关心[1] 

机构地区：[1]华中科技大学同济医学院基础医学院免疫学系,湖北武汉430030 [2]中山大学临床检验标准化研究中心,广东广州510080 [3]中山大学中山医学院微生物教研室,广东广州510080 [4]中山大学热带病防治研究教育部重点实验室,广东广州510080

出　　处：《热带医学杂志》2013年第11期1312-1315,共4页Journal of Tropical Medicine

基　　金：国家自然科学基金(81071760;30772503)

摘　　要：目的筛选与分析LNCap细胞和PC-3细胞肿瘤转移相关的差异表达基因。方法通过肿瘤转移基因芯片分析前列腺癌雄激素依赖型(ADPC)LNCap细胞以及前列腺癌雄激素非依赖型(AIPC)PC-3细胞84个肿瘤转移相关基因的表达差异。结果两个细胞株中表达差异显著基因共56个,在LNCap细胞中有41个基因下调,15个基因上调表达。结论LNCap细胞和PC-3细胞肿瘤转移相关的差异表达基因有助于研究AIPC的形成以及前列腺癌的侵袭转移机制。Objective To screening and analyze the differentially expressed tumor-metastasis related genes in LNCap cells and PC-3 cells. Methods The tumor-metastasis related gene chips were used to analyze differentially expressed 84 tumor metastasis-related genes in androgen-dependent （ADPC） LNCap cells and androgen-independent （AIPC） PC-3 cells. Results There were totally 56 differentially expressed genes expressed in the two cell lines, among which 41 were downregulated and 15 were upregulated significantly in LNCap cells. Conclusion Analysis of the differentially expressed tumor-metastasis related genes in LNCap and PC-3 cells provided could contributeion to the study of the mechanism of AIPC formation, and also the study understanding of prostate cancer inavasion and metastasis.

关 键 词：前列腺癌 AIPC ADPC 基因芯片 

分 类 号：R737[医药卫生—肿瘤]