环境内分泌干扰物对青春前期雄性大鼠性腺发育的不良影响及益肾填精方的治疗作用  

Adverse Effect of Environmental Endocrine Disruptors on Gonadal Development of Prepubertal Male Rats and Therapeutic Effect of Bushen Tianjing Recipe on It

作　　者：李祥婷[1] 蔡德培[1] 

机构地区：[1]复旦大学附属儿科医院中西医结合科,上海200032

出　　处：《中国中西医结合杂志》2013年第12期1690-1695,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30371820)

摘　　要：目的复制SD雄性大鼠由环境内分泌干扰物(environmental endocrine disruptors,EEDs)引致的性腺发育不良(gonadal dysgenesis,GD)模型,验证益肾填精方对EEDs抗雄激素活性的拮抗作用。方法 3周龄雄性SD大鼠70只随机分为7组:对照组(喂饲玉米油)、模型A组[邻苯二甲酸二(2-乙基己基)酯(di-2-ethylhexylphthalate,DEHP)500 mg/kg]、中药A组(DEHP 500 mg/kg+益肾填精方40 mL/kg)、模型B组[氯氰菊酯(Cypermethrin,CYP)80 mg/kg]、中药B组(CYP 80 mg/kg+益肾填精方40 mL/kg)、模型C组(DEHP 500 mg/kg+CYP 80 mL/kg)及中药C组(DEHP 500 mg/kg+CYP 80 mg/kg+益肾填精方40 mL/kg),每组10只。每天灌胃1次,共30天。于末次染毒24 h后处死动物,测定大鼠体重、睾丸湿重,并计算睾丸系数;测定血清睾酮(testosterone,T)水平;制备睾丸病理组织切片,光镜观察其组织学改变,电镜观察生殖细胞超微结构的变化。结果与对照组比较,各模型组及各中药组大鼠体重增加量差异无统计学意义(P>0.05),各模型组睾丸下降时间及包皮分离时间均明显延迟(P<0.01);模型A组及模型C组睾丸重量减轻,血清T降低;模型A组睾丸系数降低(P<0.01)。与相应模型组比较,各中药组睾丸下降时间及包皮分离时间均提前(P<0.01);中药A组睾丸重量、睾丸系数及T水平均增加(P<0.01),中药B组T水平明显升高(P<0.05)。结论应用DEHP剂CYP成功地复制了GD大鼠模型,证实EEDs具有显著的抗雄激素活性,并验证了益肾填精方对其抗雄激素活性具有显著的拮抗作用。Objective To verify the antagonistic effect of Bushen Tianjing Recipe （BTR） on envi- ronmental endocrine disruptors （EEDs）induced gonadal dysgenesis （GD） Sprague-Dawley （SD） male rat model. Methods Totally 70 3-week-old male SD rats were randomly divided into seven groups, i.e., the control group （fed with corn oil）, the model A group [di-2-ethylhexylphthalate （DEHP） 500 mg/kg], the CM A group （fed with DEHP 500 mg/kg ＋ BTR 40 mL/kg）, the exposed group B （fed with CYP 80 mg/kg）, the CM B group （fed with CYP 80 mg/kg ＋BTR 40 mL/kg）, the model C group [fed with DEHP 500 mg/kg ＋ CYP 80 mL/kg], the CM C group （DEHP 500 mg/kg ＋ CYP 80 mg/kg ＋ BTR40 mL/kg）, re- spectively, 10 in each group. All were administered with corresponding medication by gastrogavage, once daily, for total 30 days. Rats were killed 24 h after the last administration, and their body weight and wet testis weight were weighed. The coefficient of testis was calculated. The serum testosterone （T） level was measured by chemiluminescent immunoassay. The histopathologic tissue was prepared. The ultrastructural changes of genital cells were observed by electron microscope. Results Compared with the control group, there was no statistical difference in the body weight increase among all groups （P 〉 0.05）. The time of testicular descent and preputial separation were significantly delayed in each exposed group （P 〈0.01）. In the exposed group A and the exposed group C, the wet weight of the testes was reduced and serum T level decreased （P 〈0.01 ）. The coefficient of testis significantly decreased in the exposed group A （P 〈0.01 ）. Compared with corresponding model group, the time of testicular descent and preputial separation were significantly fore-laid in each corresponding CM group （P 〈0.01 ）. The weight of the testes, the coefficient of testis, and the serum T level increased in the CM A group（P 〈0.01 ）. The serum T level obviously increased in the CM B group （P

关 键 词：环境内分泌干扰物 邻苯二甲酸二(2-乙基己基)酯 氯氰菊酯 性腺发育不良 益肾填精方 

分 类 号：R285[医药卫生—中药学]