复合型AD大鼠模型脑组织、血清及牙周组织中部分炎症因子的表达  

Expression of Inflammatory Cytokines in the Brain, Blood Serum and Periodontal Tissue in Alzheimer＇s Disease Rat Model Induced by a Combined Treatment Procedure

作　　者：尹秀华 姜珍[1,2] 刘天云[1] 潘平[1] 秦婷[1] 夏春林[1] 孙茂民[1,2] 

机构地区：[1]苏州大学医学部解剖学教研室暨细胞神经生物学研究室,苏州215213 [2]苏州大学医学部实验动物中心,苏州215213

出　　处：《实验动物与比较医学》2013年第6期439-443,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的初步探讨β-淀粉样蛋白1-42（Aβ1—42）及D-半乳糖（D—gal）联合构建的复合型阿尔茨海默病（AD）模型中枢神经、血清及牙周组织的炎症因子的表达。方法采用24只2月龄雄性SD大鼠，共分2组，腹腔注射及侧脑室注射生理盐水的对照组，腹腔注射D—gal联合侧脑室注射A13—42建立的复合型AD模型组。Moms水迷宫实验检测大鼠的学习记忆能力，HE染色观察神经元数量和形态变化，ELISA方法检测脑组织各部分（皮质、海马）、血清及牙周组织中炎症因子（CRP，IL-1，IL-6，TNF-α。结果与对照组相比，复合型AD模型组大鼠海马、皮质中神经元形态皱缩，数量减少；CRP，IL-1，IL-6，TNF-α表达水平升高，血清和牙周组织中的CRP，IL-1，IL-6，TNF-α表达水平也升高〈0．01）。结论建立复合型AD模型，可较全面地模拟中枢神经炎症；牙周炎症可能参与了AD的发病进程。Objective To establish rat Alzheimer＇s Disease by a combined treatment with β Amyloid proteinl-42 （Aβ1-42） and D-galactose （D-gal） andinvestigate the levels of CRP, IL-1, IL-6, TNF-α in the brain, blood serum and periodontal tissue. Methods Twenty-four two-month old SD male rats were randomly divided into two groups： peritoneal injection with D-gal combined with lateral ventricle injection with Aβ1-42 to establish AD animal model, and injection of physiological saline served as the control group. The learning and memory function of the rats were evaluated by Morris water-maze test, the number and morphology of neuron in hippocampus and cortex were observed, and the expressions of CRP, IL-1, IL-6, TNF-α in the brain （hippocampus and cortex）, serum and periodontal tissue were measured by ELISA. Results Comparing with the control group, the number of neurons stained in AD animal model reduced significantly （P〈0.05）, but no morphological change was observed. The expression of CRP, IL-1, IL-6, TNF-α in the brain （hippocampus and cortex）, serum and periodontal tissue were increased in model group. Conclusion This model can better imitate inflammation in central nerve system; periodontal inflammation may involve in the progress of AD.

关 键 词：D-淀粉样蛋白 D-半乳糖 阿尔茨海默病 炎症因子 SD大鼠 

分 类 号：R-332[医药卫生] R749.16