结直肠癌组织异常表达miRNA的鉴定  

Identification of Aberrantly Expressed miRNAs in Colorectal Cancer

作　　者：汤文涛[1] 朱云祥[1,2] 傅佶泓[2] 崔龙[2] 

机构地区：[1]扬州大学第二临床医学院普外科,江苏扬州225000 [2]上海交通大学附属新华医院结直肠外科,上海200092

出　　处：《现代生物医学进展》2013年第31期6008-6010,共3页Progress in Modern Biomedicine

基　　金：中国博士后科学基金面上项目(2012M511109)

摘　　要：目的:筛选结直肠癌组织异常表达的miRNAs。方法:采用Agilent基因芯片(V12.0)分析结直肠癌组织及其配对正常粘膜组织间差异表达的miRNAs,MiRNAs错误发生率(FDR)<0.05和微矩阵显著性分析(SAM)q值<0.05为差异显著。结果:鉴定出结直肠癌中32个差异表达的miRNAs,显著上调和下调各16个。实时定量PCR(RT-qPCR)证实基因芯片中4个表达上调的miRNAs在结直肠癌组织中也显著上调。结论:MiRNA基因芯片鉴定出了结直肠癌组织一系列新的差异表达的miRNAs。Objective： To identify aberrantly expressed miRNAs in colorectal cancer. Methods： Agilent microarray（V12.0）was conducted to analyze the miRNAs expression between colorectal cancer tissues and matched normal mucosal tissues. MiRNAs with false discovery rate （FDR）〈0.05 and q-value〈0.05 for Significant Analysis of Microarray （SAM） were identified as differentially expressed genes. Results： Thirty-two aberrantly expressed miRNAs were identified including sixteen up-regulated and sixteen down-regulated. 4 up-regulated miRNAs identified by miRNA microarray had up-regulation in colorectal cancer by Real-time quantitative PCR （RT-qPCR）. Conclusion： A number of new, aberrantly expressed miRNAs in colorectal cancer were identified.

关 键 词：结直肠癌 基因芯片 MIRNAS 

分 类 号：R735.3[医药卫生—肿瘤]