B7-1基因转染小鼠EL-4淋巴瘤细胞体外诱导免疫小鼠脾细胞产生的白细胞介素-2  

IL-2 secreted by splenocyte from the immunized mice mixed culture with EL-4B7-1+ cells

作　　者：武爱民[1] 王克玉[2] 

机构地区：[1]山东工业大学科研处,山东济南250061 [2]山东医科大学附属医院皮肤科,山东济南250012

出　　处：《泰山医学院学报》2000年第4期257-258,共2页Journal of Taishan Medical College

基　　金：国家自然科学基金!(NO 3 9470 65 9)

摘　　要：目的　研究B7 1基因转染小鼠EL 4淋巴瘤体外诱导免疫小鼠脾细胞产生的白细胞介素 2。方法　以CTLL 2细胞检测EL 4B7 1+ 免疫小鼠的脾细胞与瘤细胞共育的MLTC培养上清中的IL 2活性。结果　EL 4B7 1+免疫小鼠的SPL经瘤细胞在体外刺激可诱生出明显的IL 2活性 ,而EL 4免疫小鼠的SPL则无此作用 ,两者具有较明显的差异 (P <0 0 1)。结论　B7 1基因转染小鼠EL 4淋巴瘤可在体外诱导免疫小鼠脾细胞产生较高水平的白细胞介素 2。Objective: To observe the level of IL 2 secreted by splenocytes of EL 4B7 1 + mixed culture immunized mice. Methods: CTLL 2 cells were used to detect the productivity of IL 2 secreted from splenocytes of EL 4B7 1 + cells mixed culture immunized mice. Results: The level of the production of IL 2 by splenocytes of EL 4B7 1 + mixed culture immunized mice was higher that of IL 2 produced by splenocytes of EL 4 immunized mice (P<0 01) Conclusion: A high level of IL 2 can be secreted by splenocytes of EL 4B7 1 + cells mixed culture immunized mice.

关 键 词：B7-1基因 共刺激分子 IL-2 EL-4 淋巴瘤细胞 

分 类 号：R730.2[医药卫生—肿瘤]