正交试验法优化朝鲜蓟中总酚酸的提取工艺  

Optimization of Extraction Conditions for Phenolic Acid of Cynarascolymus L. by Orthogonal Design

作　　者：赵友谊[1] 王奇志[1] 张建华[1] 冯煦[1] 王鸣[1] 

机构地区：[1]江苏省中国科学院植物研究所江苏省药用植物研究开发中心,江苏南京210014

出　　处：《食品研究与开发》2013年第20期15-17,共3页Food Research and Development

基　　金：江苏省盐土生物资源研究重点实验室开放课题(JKLBS2012011);江苏省药用植物研究开发中心开放基金(No.201202)

摘　　要：以咖啡酸含量为指标,采用L9(34)正交试验,考察提取溶媒、液料比、提取次数、提取时间因素对朝鲜蓟中黄酮及酚酸提取率的影响,研究朝鲜蓟黄酮及酚酸类成分的最佳提取工艺。最佳提取工艺为60%乙醇,液料比10∶1(mL/g),提取3次,每次2 h。本提取工艺方法简单、合理,是朝鲜蓟黄酮及酚酸类成分的最佳提取工艺。The effect of the concentration of ethanol, volume of solvent, extraction times and extraction time duration were investigated by the orthogonal design with the content of total phenolic acid as the chemical reference. To study the optimum extraction process of phenolic acid from the Cynarascolymus L. The optimum extraction condition was established as follows： ethanol concentration 60 %, liquid ratio 10 ： 1 （mL/g）, extracting 2 hours for three times. The method was simple and feasible, and is the optimum extraction process of the total flavonoids and phenolic acid from Cynarascolymus L..

关 键 词：朝鲜蓟 黄酮 酚酸 咖啡酸 正交试验 

分 类 号：TQ914[化学工程]