淫羊藿总黄酮的生物转化过程分析  

Analysis on biotransformation of Epimedium brevicornu flavonoids

作　　者：高霞[1,2] 刘璇[1,2] 陈彦[1] 王莹[1] 贾晓斌[3] 

机构地区：[1]江苏省中医药研究院中药新型给药系统重点实验室,国家中医药管理局中药口服制剂释药系统重点研究室,江苏南京210028 [2]南京中医药大学,江苏南京210046 [3]江苏省中医药研究院中药新型给药系统重点实验,室国家中医药管理局中药口服制剂释药系统重点研究室,江苏南京210028

出　　处：《中国中药杂志》2013年第23期4079-4083,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173557);江苏省中医药领军人才专项(LJ200913);江苏省“333人才培养工程”项目(BRA2011219)

摘　　要：该文旨在研究淫羊藿总黄酮在体外水解酶作用下的生物转化过程。主要采用蜗牛酶水解淫羊藿总黄酮，用HPLC测定总黄酮中主要黄酮的转化。数据结果显示已知主要黄酮成分淫羊藿苷，朝藿定A，朝藿定B，朝藿定C都在1～2h内分别完全转化为宝藿苷I，箭藿苷A，箭藿苷B和鼠李糖基淫羊藿次苷Ⅱ，随着水解时间的延长，各转化产物被继续水解。因此可得出结论，蜗牛酶在37℃，pH6．0的Hank’s平衡盐溶液中，2h内能将淫羊藿主要总黄酮转化为次级苷或苷元，且其酶解产物与肠道代谢产物相一致。This study aims to investigate the biotransformation of Epimedium brevicornu flavonoids under the effect of hydrolytic enzymes in vitro. Snailase was mainly used to hydrolyze E. brevicornu flavonoids, and HPLC was used to determine the content of the main flavonoids in E. brevicornu flavonoids. The data results showed that the main known flavonoids included icariin, epimedin A, epi- mendin B and epimendin C, which were completely transformed into baohuoside I, sagittatoside A, sagittatoside B and 2＂-O-rhamnosyl- icariside II in 1-2 h, respectively. Their transformed products were continuously hydrolyzed over time. In conclusion, snailase could transform E. brevicornu flavonoids into secondary glycoside or aglycone under 37 ℃ in pH 6.0 HBSS balanced salt solution in 2 h. Mo- reover, its enzymatic hydrolvsates were consistent with intestinal metabolites.

关 键 词：淫羊藿总黄酮 蜗牛酶 生物转化 

分 类 号：R284[医药卫生—中药学]