滨梅茎段组培污染率控制方法的研究  

Studies on the Methods for Control of Contamination in Tissue Culture of Prunus maritima Stems

作　　者：燕志 张焕仕[2] 王猛[1] 张浩[1] 钦佩[3] 

机构地区：[1]南京大学连云港高新技术研究院,连云港222000 [2]南京野生植物研究院 [3]南京大学盐生植物实验室

出　　处：《中国南方果树》2013年第6期9-13,共5页South China Fruits

基　　金：中国博士后科学基金面上资助项目(2012M511728);江苏省连云港市科技攻关项目(CG1143)资助

摘　　要：以当年生滨梅茎段为试材,针对在组培过程中滨梅茎段易感染外生菌和内生菌的问题进行了污染率控制方法的探索。结果表明:(1)不同的消毒试剂中,0.1%升汞消毒效果最好;(2)不同消毒试剂最优组合为75%酒精+0.1%升汞,可以将外生菌染菌率控制在8.25%,死亡率在18.20%;(3)最优消毒试剂组合的最佳消毒时间为先用75%酒精消毒30秒,再用0.1%升汞消毒5分钟;(4)抗生素添加试验中,添加浓度为0.5%制霉菌素可使内生菌染菌率减少到3.2%,死亡率减少到9.2%,无菌苗获得率为81.3%。In the tissue culture of Prunus maritima Marshall stem, explants may be contaminated by bacteria and endophytes. The methods for controlling contamination were studied with one--year old stem segments of Prunus maritima. The results showed that： （1） 0. 1% HgC12 showed the best control effect. （2） The best combination of disinfectant was 0. 1% HgC12 ＋ 75% alcohol, which ef- fectively controlled contamination to the infection rate of bacteria at 8. 25% and mortality rate at 18. 20%. （3） The best disinfection time with the best disinfectant combination was 75% alcohol for 30 seconds, and then 0. 1% HgCl2 for 5 minutes. （4） In the antibiotic test, after adding 5% nysta- tin dihydrate, the contamination rate of endophytes could be further reduced to 3. 2%, the mortality rate reduced to 9.2% and the aseptic seedling rate was 81.3%.

关 键 词：滨梅 组织培养 外生菌 内生菌 

分 类 号：X173[环境科学与工程—环境科学] S66[农业科学—果树学]