SMMC-7721细胞诱导耐药培养中P-糖蛋白变化  

The study on the Dynamic Changes of P-glycoprotein in Drug-Resistant Inducing Culture of SMMC-7721 Cells

作　　者：魏志霞[1] 田韧[1] 杨劲松[1] 

机构地区：[1]江苏省南京医科大学附属南京第一医院,210006

出　　处：《齐齐哈尔医学院学报》2000年第6期604-605,共2页Journal of Qiqihar Medical University

摘　　要：目的　研究SMMC - 772 1细胞诱导耐药培养中P -糖蛋白变化。方法　用MTT法检测常用化疗药物对SMMC - 772 1和SMMC - 772 1/ADM细胞的毒性 ;用流式细胞仪检测SMMC - 772 1/ADM细胞P -糖蛋白表达及细胞内柔红霉素的浓度。结果　随着培养液中阿霉素浓度的递增 ,SMMC- 772 1/ADM细胞内柔红霉素的积聚减少 ,P -糖蛋白表达有逐步增加的趋势。结论　P -糖蛋白的过度表达可能是形成SMMC - 772 1细胞多药耐药的重要原因。Purpose To investigate the changes of P-glycoprotein in drug-Resistant Inducing Culture of SMMC-7721 Cells. Methods The expression of P-glycoprotein of SMMC-7721/ADM cells and the concentration of DNR in cells were studied by flow cytometric analysis .The cytotoxicity of chemotherapeutic drugs to SMMC-7721 and SMMC-7721/ADM cells were detected by MTT. Results With increasing the concentration of ADM, the accumulation of DNR in SMMC-7721/ADM cells reduced, the expression of P-glycoprotein increased.Conclusions Acquisition of multidrug resistance(MDR) is often associated with overexpression of P-glycoprotein.

关 键 词：肝癌细胞 多药耐药 P-糖蛋白 柔红霉素 

分 类 号：R735.7[医药卫生—肿瘤]