利用变性梯度凝胶电泳分析正红菇菌根围土壤真菌群落多样性  

Analysis of Fungal Diversity of Russula griseocarnosa Mycorrhizosphere Soil with Denaturing Gradient Gel Electrophoresis

作　　者：肖冬来[1] 陈丽华[2] 陈宇航[1] 杨菁[1] 黄小菁[1] 

机构地区：[1]福建省农业科学院食用菌研究所,福建福州350003 [2]福建省农业科学院中心实验室,福建福州350003

出　　处：《热带作物学报》2013年第12期2508-2512,共5页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金项目(No.2011J05056);福建省农业科学院青年人才基金项目(No.2010BS-1)

摘　　要：以正红菇(Russula griseocarnosa)菌根围土壤为研究对象,通过提取土壤基因组DNA,以通用引物扩增真菌18S rRNA基因V1+V2区,将PCR产物进行变性梯度凝胶电泳(Denaturing Gradient Gel Electrophoresis),获得土壤微生物群落的DNA特征指纹图谱,并对图谱中的优势条带回收测序,通过Blast进行同源性比对并构建系统发育树,进而分析正红菇菌根围真菌群落组成及多样性。同源性比对结果表明,在回收测序的19条DGGE条带中,4条为非真菌真核生物序列,系统发育分析显示全部序列可以分为4类菌群,GroupⅠ主要为担子菌门(Basidiomycota)真菌,GroupⅡ主要为子囊菌门(Ascomycota)真菌,GroupⅢ为未知真菌,GroupⅣ主要为节肢动物门生物(Arthropoda)。In order to reveal fungal diversity of Russula griseocarnosa mycorrhizosphere soil,the total genomic DNA of soil microbial communities was extracted.Fungal 18S rRNA V1 ＋V2 region was amplified by universal primers and denaturing gradient gel electrophoresis （DGGE） was applied to obtain the DNA fingerprint profile of the soil fungal communities.Distinct DGGE bands were excised,cloned,sequenced and sequences homogy analyzed by BLAST tool.Neighbor-joining phylogenetic tree was constructed by MEGA software.The results indicated that nineteen DGGE bands were sequenced and homogenous analysis showed that four DGGE bands were assigned to non-fungal eukaryotic sequences.Phylogenetic analysis revealed these nineteen bands could be divided into four groups：Group Ⅰ to Basidiomycota,GroupⅡ to Ascomycota,GroupⅢ to unknown fungi,GroupⅣ to Arthropoda.The study provides a better understanding of the relationship between Russula griseocarnosa and mycorrhizosphere fungi.

关 键 词：正红菇 菌根围 真菌多样性 PCR变性梯度凝胶电泳 

分 类 号：S646.9[农业科学—蔬菜学]