罗格列酮对大鼠脑缺血再灌注损伤后IL-18表达的影响  

Effects of Rosiglitazone Maleate on IL-18 Expressing in Rats After Cerebral Ischemia-Reperfusion

作　　者：张宁[1] 刘元[1] 

机构地区：[1]中南大学湘雅医院神经内科,湖南长沙410008

出　　处：《中风与神经疾病杂志》2013年第11期1004-1007,共4页Journal of Apoplexy and Nervous Diseases

基　　金：湖南省自然科学基金项目(08JJ3063)

摘　　要：目的观察不同剂量过氧化物酶体增殖物受体γ(PPARγ)激动剂罗格列酮对大鼠脑缺血再灌注时炎性因子IL-18表达的影响。方法健康雄性大鼠60只,随机分为随机分为5组:假手术组(n=12),缺血再灌注模型组(n=12)以及罗格列酮0.5mg/kg、2mg/kg、5mg/kg大中小3种剂量干预组(各组n=12)。采用Longa线栓法制备大脑中动脉栓塞模型,分别于缺血即刻及缺血2h后经胃管按罗格列酮0.5mg/kg、2mg/kg、5mg/kg 3种剂量分别灌入。在缺血2h再灌注24 h后处死大鼠,分别采用HE染色观察缺血周边区病理学变化,TTC染色检测脑梗死体积,免疫组织化学法检测IL-18表达。结果与模型组相比,罗格列酮2mg/kg、5mg/kg干预组脑梗死体积及IL-18表达明显减少,差异具有统计学意义(0.260 Vs 0.452、0.240 Vs 0.452mm3,P<0.05)。结论罗格列酮能够减少脑梗死体积,其对脑缺血再灌注损伤的保护作用可能与下调IL-18表达有关。Objective To evaluate the effects of Rosiglitazone Maleate on the expressions of IL-18 after focal cere- bral ischemia-reperfusion in rats. Methods The healthy male sprague-dawley rats weighting 250 - 280g were randomly di- vided into five groups ： sham operation group（ n = 12 ）, NS control group（ n = 12 ） , Rosiglitazone maleate 0.5,2 and 5 mg ·kg-lThree dose treatment group（ every team n = 12）. Focal cerebral ischemia was induced by the intraluminal suture for middle cerebral artery occlusion. Rosiglitazone maleate 0.5,2 and 5 mg · kg-1 were taken orally immediately and 2 h after MCA occlusion. The pathologic changes were observed by hematoxylin and eosin（HE） staining. TFC staining was used to measure the infarct area. The protein expression of IL-18 were measured with methods of immunohistochemistry. Results Compared to control group, the infarct area and the expressing of IL-18 in RSG2,5mg o kg-1 dose group was significantly re- duced （0.260VS0.452,0. 240VS0. 452mm3 ,P 〈 0.05 ）. Conclusion PPARγ ligand rosiglitazone could protect brain from ischemic injury by inhabiting the expressing of IL-18 after an 24 h MCA occlusion.

关 键 词：缺血再灌注 罗格列酮 IL-18 

分 类 号：R743.3[医药卫生—神经病学与精神病学]