阿司匹林对大鼠醋酸胃溃疡愈合的影响  

Effect of aspirin on healing of gastric ulcer induced by acetic acid in rats

作　　者：王国忠[1] 肖薇[1] 李波[1] 

机构地区：[1]齐齐哈尔医学院病理生理教研室,黑龙江齐齐哈尔161006

出　　处：《中国医院药学杂志》2013年第23期1925-1928,共4页Chinese Journal of Hospital Pharmacy

基　　金：黑龙江省教育厅科学技术研究项目(编号:12531803)

摘　　要：目的:探讨阿司匹林(Asp)抑制胃溃疡愈合的机制。方法:用醋酸诱导大鼠胃溃疡,8 d后随机分为模型对照组、盐水对照组、阿司匹林组。第16天用免疫组化方法检测胃黏膜转化生长因子-α(TGF-α)、增殖细胞核抗原(PCNA)的表达,用Schiff-阿尓新蓝染色法检测胃黏液,并检测TGF-α、PCNA和胃黏液的积分光密度。激光多谱勒血流仪检测胃黏膜血流(GMBF)。结果:阿司匹林组的TGF-α、PCNA的积分光密度低于模型对照组和盐水对照组(P<0.01),阿司匹林组的胃黏液积分光密度低于模型对照组和盐水对照组(P<0.05),阿司匹林组的GMBF低于模型对照组和盐水对照组(P<0.01)。结论:阿司匹林抑制胃黏膜TGF-α、PCNA的表达、减少GMBF,导致胃黏液减少,抑制溃疡愈合。OBJECTIVE To explore the mechanism of aspirin（Asp） inhibiting gastric ulcer healing. METHODS Gastric ulcers were induced with acetic acid in rats, and the rats with the gastric ulcer were randomly divided into model, saline and aspirin groups after 8 day. On 16th day, the expression of transforming growth factor-α（TGF-α）, proliferating cell nuclear antigen （PCNA） were detected through immunohistochemical method in gastric mucosa. Gastric mucus was detected through Schiff- Alcian-blue staining method, and then the integral light density of TGF-α, PCNA and gastric mucus were detected. Gastric mucosal blood flow （GMBF） was detected with Laser Doppler flowmetry. RESULTS The integral light density of the TGF-α and PCNA in aspirin group was decreased compared with that of the TGF-α and PCNA in model or saline groups （P〈0.01）. The integral light density of the gastric mucus in aspirin group was decreased compared with that of the gastric mucus in model or saline groups （P〈0. 05）. GMBF in aspirin group was decreased compared with that of model or saline groups （P〈0. 01 ）. CONCLUSION Asp inhibits the expression of TGF-α and PCNA in the gastric mucosa and decreases GMBF, which leads to reducing of the gastric mucus and then inhibiting ulcer healing.

关 键 词：阿司匹林 胃溃疡 转化生长因子-Α 增殖细胞核抗原 

分 类 号：R573.1[医药卫生—消化系统]