蜂毒素对荷骨肉瘤裸鼠体瘤内皮祖细胞数量和细胞因子HIF-1α及SDF-1α的影响  

The effects of melittin on the endothelial progenitor cell count,hypoxia inducible factor-1α,stromal cell-de-rived factor-1α protein in UMR106-osteosarcoma-bearing mice

作　　者：秦刚[1] 李海东[2] 徐苏洋[2] 李玉梅[2] 孙剑[2] 陈永强[2] 

机构地区：[1]广西中医药大学第一附属医院骨三科,南宁530023 [2]上海市中医医院骨科,200071

出　　处：《广东医学》2013年第21期3226-3229,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30873275)

摘　　要：目的观察蜂毒素对荷骨肉瘤裸鼠微血管生成的影响,并探讨其作用的机制。方法建立荷骨肉瘤裸鼠模型,随机分为生理盐水组和蜂毒素低、中、高剂量组,分别予以生理盐水及蜂毒素160、320、640μg/kg的剂量局部干预瘤体。采用免疫荧光染色法、免疫组织化学染色法和显微图像分析技术检测各组荷骨肉瘤裸鼠肿瘤中内皮祖细胞(EPC)数量及缺氧诱导因子-1α(HIF-1α)、基质细胞衍生因子-1α(SDF-1α)的表达。结果与生理盐水组比较,蜂毒素低、中、高剂量组肿瘤组织中CD34/CD133双阳性细胞的数量均明显减少,HIF-1α、SDF-1α的阳性表达率均明显降低,差异有统计学意义(P<0.05)。结论蜂毒素能减少荷骨肉瘤裸鼠肿瘤组织中EPC数量,其作用机制可能与蜂毒素下调HIF-1α和SDF-1α蛋白表达有关。Objective To investigate the effects and mechanism of melittin on angiogenesis via assessment of the endothelial progenitor cell （EPC） count and the expression of hypoxia inducible factor - 1a （HIF - 1a） and stromal cell - derived factor - 1a （ SDF - 1a） in UMR106 - osteosarcoma - bearing mice. Methods The UMR106 - osteosarcoma - bearing mouse models were established and randomly divided into physiological saline （NS） group, melittin low dose （MLD） group, melittin medium dose （MMD） group and melittin high dose （MHD） group; and treated with NS, MLD, MMD and MHD, respectively. By immune fluorescence staining method, immunohistochemical method and microscopic image analysis technique, the EPCs quantity and the expression of HIF - 1a and SDF - 1a protein were assessed. Results The quantity of CD34/CD133 double positive cells of all melittin groups were significantly reduced when comparing with NS group （ P 〈 0. 01）. Significant down - regulation in HIF - 1 a and SDF - 1 a in all melittin groups was also revealed （P 〈0. 05）. Conclusion Melittin can reduce the EPCs count in UMR106 -osteosarcoma -bearing mice, probably via the down - regulation of HIF - 1a and SDF - 1a.

关 键 词：蜂毒素 骨肉瘤 内皮祖细胞 血管生成 缺氧诱导因子-1α 基质细胞衍生因子-1Α 

分 类 号：R739.41[医药卫生—肿瘤]