阿霉素加热化疗对人肝癌细胞耐药模型-7721/Adm多药耐药性的影响  

Effect of thermochemotherapy of adriamycin on multidrug-resistant of human multidrug-resistant hepatocellular carcinoma cell line (7721/Adm)

作　　者：张洪新[1] 刘燕[2] 郭卫平[1] 王执民[1] 王义清[1] 高巍[2] 李文献[1] 倪代会[1] 曹伟[1] 贺朝 

机构地区：[1]西安第四军医大学唐都医院介入放射科,710038 [2]西安第四军医大学唐都医院,710038 [3]陕西省第二纺织医院放射科

出　　处：《中华物理医学与康复杂志》2000年第6期366-369,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：陕西省攻关课题资助项目部分内容!(97Y0 3);第四军医大学科技创新工程资助项目!(CX99A0 )

摘　　要：目的　探讨阿霉素 (ADM )化疗与 43℃加热化疗对人肝癌细胞 772 1(以下简称 772 1细胞 )和耐药人肝癌细胞模型 772 1/Adm(以下简称 772 1/Adm细胞 )的细胞毒作用。方法　以体外培养的人肝癌细胞 772 1和我们培养建立的人肝癌细胞模型 772 1/Adm为研究对象 ,采用水浴加温法 ,体外细胞毒试验(MTT法 ) ,观察ADM化疗与加热化疗对细胞的生长抑制规律的影响 ;采用流式细胞技术检测加热对772 1细胞和 772 1/Adm细胞胞内阿霉素浓度的影响。结果　① 772 1/Adm细胞对阿霉素的敏感性明显低于 772 1细胞 ;②加热可以明显提高两种细胞对阿霉素的敏感性 ;③流式细胞检测显示加热可明显提高这两种细胞内的阿霉素的浓度 ,尤其是 772 1/Adm细胞内的阿霉素浓度。结论　加热可以显著提高人肝癌细胞 772 1和耐药人肝癌细胞模型 772Objective To comparatively study the cytotoxic effects of chemotherapy and thermo chemotherapy of adriamycin (ADM) on human hepatocellular carcinoma cell line (7721) and human multidrugresistant hepatocellular carcinoma cell line (7721/Adm). Methods By means of warm water bath and MTT assay, the inhibition pattern of cell growth was observed. The effects of hyperthermia on intracellular concentration of ADM of 7721 and that of 7721/Adm were detected by flow cytometry. Results ①The sensitivity of 7721/Adm to ADM was significantly lower than that of 7721; ② Hyperthermia remarkably increased the sensitivity of 7721 and 7721/Adm to AMD; ③Hyperthermia increased the intracellular concentration of ADM in those two sorts of cells mentioned above, especially in 7721/Adm. Conclusion Hyperthermia can increase the sensitivity of 7721 and 7721/Adm to ADM.

关 键 词：阿霉素 热化疗 人肝癌细胞 耐药性 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]