食管癌肿瘤浸润淋巴细胞体外增殖和表型变化的研究  

STUDY ON THE PROLIFERATION 2ND PHENOTYPES OF TILS FROM ESOPHAGEAL CANCER IN VITRO

作　　者：任德莲[1] 严鹏科 钟德生 谢建强 徐晓玉[1] 戴天阳[1] 李成文[1] 李程旭[1] 

机构地区：[1]泸州医学院微生物学与免疫学教研室,646000 [2]攀钢集团职工总医院胸心外科

出　　处：《泸州医学院学报》1999年第5期375-378,共4页Journal of Luzhou Medical College

基　　金：四川省中医管理局科研基金!96025

摘　　要：目的：寻找新的TIL刺激物，摸索迅速解除TIL抑制状态和提高扩增效率的最佳条件。方法：通过黄芪、干地黄水提物联合细胞因子，采用3H－TdR掺入法及流式细胞仪对9例食管癌TIL的增殖及表型进行了分析。结果：在促增殖作用方面，黄芪联合IL-2、IL-2＋IL－4、IL－2＋TNF－a均较单用其细胞因子有明显增强作用。而干地黄对TIL增殖无明显协同作用。食管癌TIL表型分析发现CD4+T细胞为主，诱导后CD4+T细胞明显增多，CD4/CD8比值由2．8：1上升至9．9：1。结论：黄芪有明显协同细胞因子促进食管癌TIL增殖的作用，激活的食管癌TILCD4+T细胞占绝对优势。Objective: To find out new activator of tumor infiltrating lymphocytes from esophageal cancer, to relieve the repression of TILs and to explore the best condition of TIL proliferation. Methods: TILs were isolated from 9 patients with esophageal cance. With water extract of Astragalus Membranaceus(Aw) or Rehamnnia Glutinosa (RG) combining cytokines the proliferation and phenotypes ot the TILs were investigated by [3H] thymidine incorporation method and flow cytometry. Result: The proliferation of TILs under AM combining with IL-2、IL-2+IL-4、IL-2+TNF-a were significantly stronger than those of the cytokines alone(P<0.05 or P<0.01). However RG combining cytokines showed no effect. It was found that the phenotypes of the TILs were mainly composed of CD4+T cell. the activation CD4+T cell evidendy increased, and the ratio of CD4/CD8 was increased from 2.8:1 to 9.9:1. Conclusion: The results suggested that the AM combining cytokines have the function of promoting proliferation of the TILs. The CD4+T cell is predominant in the astivated TILs.

关 键 词：表型 食管癌 肿瘤浸润淋巴细胞 体外增殖 

分 类 号：R735.1[医药卫生—肿瘤]