上海地区幽门螺杆菌菌株cag致病岛基因cagA、cagE、cagT检出率及临床意义  

Prevalence of cagA, cagE and cagT genes in cag pathogenicity island of Helicobacter pylori strains isolated from Shanghai patients and their clinical implications

作　　者：李晓波[1] 刘文忠[1] 徐蔚文[1] 施尧[1] 萧树东[1] 

机构地区：[1]上海第二医科大学仁济医院,上海市消化疾病研究所,200001

出　　处：《中华消化杂志》2000年第6期371-373,共3页Chinese Journal of Digestion

摘　　要：目的　检测上海地区幽门螺杆菌 (Hp)分离株cag致病岛 (PAI)中cagA、cagE、cagT基因 ,初步探讨cagPAI的完整性及其与胃十二指肠疾病的关系。 方法　用多聚酶链反应 (PCR)技术扩增和检测了 99株从 17例慢性浅表性胃炎、2 1例慢性萎缩性胃炎、19例胃溃疡、2 3例十二指肠溃疡和 19例胃癌中分离的Hp菌株的cagA、cagE、cagT基因。 结果　cagA、cagE和cagT的总检出率分别为 84.8%(84/ 99)、99.0 % (98/ 99)和 84.8% (84/ 99) ,各基因检出率在各种胃十二指肠疾病患者分离的Hp菌株之间差异无显著性 (均P >0 .0 5 )。 98株cagE阳性的菌株中 ,有 14株cagA阴性。结论　上海地区分离的Hp菌株绝大多数可能具有完整的cagPAI,其完整性与其感染后的临床结局不相关 。Objective To investigate the prevalence of the cagA, cagE and cagT, representative genes of cag pathogenicity island (PAI) in H. pylori strains isolated from patients with various gastroduodenal diseases in Shanghai and the relationship between the presence of these genes and gastroduodenal diseases. Methods 99 H. pylori strains isolated from 17 chronic superficial gastritis, 21 chronic atrophic gastritis, 19 gastric ulcer, 23 duodenal ulcer and 19 gastric cancer were studied for the prevalence of cagA, cagE and cagT genes by polymerase chain reaction (PCR). Results The total prevalences of cagA, cagE and cagT genes were 84.8%, 99.0% and 84.8%, respectively. There was no significant difference in the prevalence of all genes tested in H. pylori isolated from different gastroduodenal diseases (all P>0.05). Of 98 cagE positive isolates, 14(14.3%) were cagA negative. Conclusion Most of the H. pylori isolates in Shanghai region may have intact cag PAI. cagE, could be taken as the marker for the presence of cag PAI. There is no correlation between the integrality of cag PAI and the clinical outcome of H. pylori of infection.

关 键 词：幽门螺杆菌 CAG致病岛 胃疾病 十二指肠疾病 上海 

分 类 号：R573[医药卫生—消化系统] R378.2[医药卫生—内科学]