人胃癌细胞系环氧合酶的表达、分布及其意义  

Expression and distribution of COX-2 in human gastric cancer cells

作　　者：李玲[1] 吴开春[1] 聂永战[1] 吴汉平[1] 王春梅[2] 樊代明[1] 

机构地区：[1]第四军医大学西京医院全军消化病研究所，710032 [2]第四军医大学中心实验室

出　　处：《中华消化杂志》2000年第6期374-377,共4页Chinese Journal of Digestion

基　　金：国家自然科学基金资助(39870230)

摘　　要：目的　检测环氧合梅 (cyclooxygenase,COX)在人胃癌细胞系的表达并了解其异构酶的亚细胞定位。方法　应用免疫细胞化学及RT PCR方法研究COX在人胃癌细胞系的表达 ,用免疫荧光双标记结合激光共聚焦显微镜技术 (LSCM)显示COX在细胞内的分布。结果　在蛋白质水平胃癌细胞MKN45、SGC790 1及AGS表达COX 2和COX 1,而MGC80 3不表达COX 2 ,COX 1为弱阳性。在蛋白质和mRNA水平上COX 2的表达均明显高于COX 1。用LSCM进一步对荧光强度进行扫描定量证实 ,COX 2表达强于COX 1,COX 2的象素密度高峰值为 5 0～ 70 ,COX 1为 10 ;同时发现COX 1仅表达于胞浆 ,而COX 2表达于胞浆和胞核。结论　胃癌细胞存在COX 2和COX 1的表达 ,且COX 2的表达水平上调 ;Objective To detect the expression of cyclooxygenase(COX) in human gastric cancer cell lines and its subcellular location of the isoforms. Methods Immunohistochemistry、RTPCR combined with laser scanning cofocal microscopy (LSCM) were used to investigate expression and distribution of COX. Results Positive staining of COX2 and COX1 protein was seen in human gastric cancer cell line MKN45、SGC7901 and AGS. However, the COX2 staining was absent and COX1 staining was weak in MGC803; though their mRNA in all the four cell lines. When compared to COX1,COX2 showed a stronger signal at both protein and mRNA levels of the gastric cancer cell lines, which was confirmed by double labeling and LSCM. The quantitative analysis of fluorescein intensity indicated the pixel intensity peak of COX2 reached 50～70, while COX1 only 10. LSCM also showed that COX2 was both cytoplasmic and nuclear envelope staining but COX1 only in cytoplasm. Conclusions In human gastric cancer, there is stronger expression of COX2 than COX1, and different distribution of the two isoforms implies their distinct roles in cell function.

关 键 词：胃癌 环氧合酶 表达 分布 胃癌细胞系 CDNA PCR 

分 类 号：R735.2[医药卫生—肿瘤]