铁皮石斛中总酚的含量测定  

Determination of Total Phenols in Dendrobii Officinalis Caulis

作　　者：李娟[1] 麻晓雪[1] 李顺祥[1] 张家曾 

机构地区：[1]湖南中医药大学中药现代化教育厅重点实验室,长沙410208

出　　处：《中国实验方剂学杂志》2013年第24期60-62,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖南省科技计划重大专项项目(2010FJ1010-3);国家中医药管理局“药用植物学”重点学科课题(国中医药发[2009]30号);湖南“中药学”重点学科(湘教通[2011]76号);湖南省高校科技创新团队项目(湘教通[2010]212号)

摘　　要：目的： 建立铁皮石斛总酚含量的快速测定方法。方法： 以阿魏酸为对照品，采用福林酚比色法测定铁皮石斛中总酚含量。通过单因素试验筛选检测波长、显色时间和温度等显色条件；以总酚含量为指标，通过L9（34）正交试验考察乙醇体积分数、料液比、提取时间和提取次数对总酚提取工艺的影响，确定含量测定中供试品溶液的制备方法。结果： 显色条件为检测波长760 nm，福林酚试剂用量3.5 mL，10%碳酸钠溶液用量7 mL，显色温度20-30℃，避光反应时间0.5 h。铁皮石斛总酚的提取工艺为加40倍量70%乙醇提取1 h。总酚含量测定的线性范围3.168-9.504 mg·L-1，平均加样回收率97.9%，RSD 1.53%。结论： 建立的含量测定方法操作简便、快速、准确、稳定、重复性好，可用于铁皮石斛中总酚含量的测定。Objective： To establish a rapid determination of total phenols in Dendrobii Officinalis Caulis. Method： Folin-Ciocalteu Colorimetry was adopted to determine the content of total phenols in Dendrobii Officinalis Caulis by taking ferulic acid as a reference.Single factor tests were adopted to screen chromogenic conditions,such as detection wavelength,chromogenic time and temperature,et al;With the content of total phenols as index,effects of ethanol concentration,solid-liquid ratio,extraction time and times on extraction technology of total phenols were investigated by L9（34） orthogonal test,in order to define preparation method of the test solution for determination. Result： Chromogenic conditions were as follows：detection wavelength 760 nm,the amount of Folin-Ciocaileu reagent 3.5 mL,the amount of 10% sodium carbonate solution 7 mL,chromogenic temperature 20-30℃,dark reaction time 0.5 h.Optimum extraction of total phenols from Dendrobii Officinalis Caulis was as following：extracted 1 h with 40 times the amount of 70% ethanol.The concentration of ferulic acid had a good linear relationship in range of 3.168-9.504 mg·L-1,average recovery was 97.9% with a corresponding RSD of 1.53% （n=6）. Conclusion： This determination method was simple,rapid,accurate,stable and reproducible for determining the content of total phenols in Dendrobii Officinalis Caulis.

关 键 词：铁皮石斛 总酚 福林酚 含量测定 正交试验 单因素试验 

分 类 号：R284.1[医药卫生—中药学]