欧洲鹅耳枥种子层积过程中贮藏物质及酶活性的变化  

Storage substance content and corresponding enzyme activity during the stratification of Carpinus betulus seeds

作　　者：祝遵凌[1,2] 林庆梅[2] 许园园[2] 

机构地区：[1]南京林业大学艺术设计学院,江苏南京210037 [2]南京林业大学风景园林学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2013年第6期157-160,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省科技支撑计划(BE2012345);国家林业局"948"项目(2011-4-44);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：对欧洲鹅耳枥种子进行变温层积处理,测定种子发芽率,淀粉、可溶性糖、蛋白质、粗脂肪含量及相关酶的活性,研究欧洲鹅耳枥种子萌发前层积过程中主要贮藏物质的含量以及相应酶活性的动态变化。结果表明:在变温层积过程中,欧洲鹅耳枥种子中淀粉、粗脂肪逐渐减少,可溶性蛋白含量先增加后减少,层积结束时较对照分别减少了39.7%、92.2%、2.3%;可溶性糖含量明显增加,较对照增加了129%;相应酶活性呈增加趋势,淀粉酶、酸性磷酸酯酶及过氧化物酶活性较对照分别增加了550%、228%、67%;变温层积150 d后种子发芽率大幅度升高。说明变温层积能有效解除欧洲鹅耳枥种子的休眠,贮藏物质不断分解代谢,为种子萌发做准备。In order to determine the changes in seed germination, biochemical composition and enzyme activity after al- ternative stratification, Carpinus betulus seeds were investigated. The results indicated that the content of starch and crude fat all gradually declined, the soluble protein climbed up first, but then declined, they respectively reduced 39. 7%, 92. 2%, 2.3% after alternative stratification, compared with CK; the content of soluble sugars obviously in- creased by 129%, the corresponding enzyme activity had an increase trendency, increased by 550%, 228%, 67% , compared with CK. After being laminated 150 d, the germination percentage increased greatly. The result indicated that alternative stratification could effectively break dormancy of Carpinus betulus L. seeds and the stored substance was con- tinuously catabolism to prepare for the seeds germination in the process of alternative stratification.

关 键 词：欧洲鹅耳枥 种子层积 淀粉 可溶性糖 粗脂肪 可溶性蛋白 酶活性 

分 类 号：S722[农业科学—林木遗传育种]