雌性SD大鼠自发性乳腺肿瘤发生情况及其生存分析  

Occurrence of spontaneous mammary neoplasm in female SD rats and their survival analysis

作　　者：马超亚[1] 宋向荣[1] 高洪彬[1] 罗雪婷[1] 伍津[1] 郑杰蔚 

机构地区：[1]广东省职业病防治院毒理实验所,广东省职业病防治重点实验室,广东广州510300

出　　处：《中国职业医学》2013年第6期505-510,共6页China Occupational Medicine

基　　金：国家自然科学基金委员会青年科学基金(81202246);广东省职业病防治重点实验室(2012A061400007)

摘　　要：目的观察无特定病原体(SPF)级雌性SD大鼠自发性乳腺肿瘤发生情况,探讨其生存的影响因素。方法对2008年1月至2012年5月间进行的5个慢性毒性合并致癌试验中对照组的400只SPF级雌性SD大鼠自发性乳腺肿瘤的发生情况进行分析。选取发生自发性乳腺肿瘤的大鼠36只,按照肿瘤性质分为良性乳腺肿瘤组18只(乳腺纤维腺瘤11只,乳腺腺瘤7只)和恶性乳腺肿瘤组18只(均为乳腺癌),计算肿瘤系数,采用免疫组织化学法检测其肿瘤细胞的增殖细胞核抗原(PCNA)蛋白的表达情况,分析大鼠生存的影响因素。结果 400只大鼠中有186只检出自发性乳腺肿瘤,肿瘤发生率为46.50%;其中173只发生乳腺纤维腺瘤,20只发生乳腺腺瘤,19只发生乳腺腺癌,肿瘤发生率分别为43.25%、5.00%和4.75%。恶性乳腺肿瘤组体质量低于良性乳腺肿瘤组(中位数:638.80 vs 571.30 g,P<0.05),PCNA蛋白阳性表达率高于良性乳腺肿瘤组(72.22%vs 22.22%,P<0.01)。2组肿瘤质量和肿瘤系数分别比较,差异均无统计学意义(P>0.05)。Cox比例风险回归模型分析显示肿瘤性质、肿瘤系数及PCNA蛋白表达强度是乳腺肿瘤生存的独立影响因素(P<0.05)。结论乳腺肿瘤是雌性SD大鼠常见的自发性肿瘤,恶性肿瘤、肿瘤系数越小、PCNA蛋白阳性表达强度越强者,大鼠生存时间相对较短,预后较差。Objective To observe the occurrence of spontaneous mammary neoplasm in specific pathogen free female spra- gne-dawley （SD） rats, and to discuss the impact factors of the survival. Methods We analyzed the features of the sponta- neous mammary neoplasm of 400 female SD rats which were used as the the control groups in 5 2-year chronic toxicity and eareinogenicity tests from January 2008 to May 2012. Thirty-six cases suffering from spontaneous mammary neoplasm were divided into 2 groups： benign group （11 cases of fibroadenoma, 7 cases of mammary adenoma） and malignant group （18 cases of mammary adenoearcinomas）. In order to analyze the impact factors of the survival, the tumor coefficient was cal- culated and the expression of proliferating cell nuclear antigen （PCNA） was detected by immunohistochemistry. Results Among these 400 female SD rats, 186 rats were observed having spontaneous mammary neoplasm with the incident rate of 46. 50% （186/400）. In these neoplasm cases, 173 cases were mammary fibroadenomas, 20 cases of mammary adenomas, 19 cases of mammary adenocarcinomas, and the incident rates were 43.25%, 5.00% and 4. 75% respectivly. Body weight of the malignant group was lower than that of the benign group （median： 638.80 vs 571.30 g,P 〈 0. 05 ）. The positive ex- pression rate of PCNA in malignant group was much higher than that in benign group （72. 22% vs 22. 22% ,P 〈0. 01 ）. Comparison of the tumorous weight and the tumor coefficient between the 2 groups demonstrated that no statistic significant differences existed （ P 〉 0. 05 ）. The results of Cox proportional-hazards regression analysis showed that the nature of the tumor, tumor coefficients and the expression of PCNA were the independent influencing factors for the overall survival （P 〈 0. 05 ）. Conclusion Mammary tumor was the most common spontaneous neoplasm in female SD rats. The less tumor coe- fficients of the malignant tumors and the higher expression of PCNA, the shorter of the survival and the worse

关 键 词：自发性乳腺肿瘤 SD大鼠 良性 恶性 增殖细胞核抗原 生存分析 

分 类 号：R737.9[医药卫生—肿瘤] R13[医药卫生—临床医学]