液相色谱-同位素比质谱法同时测定葡萄酒中甘油和乙醇的δ^(13)C值  

Simultaneous determination ofδ^(13)C values of glycerol and ethanol in wine byliquid chromatography coupled with isotope ratio mass spectrometry

作　　者：李学民[1] 贾光群[1] 曹彦忠[1] 张进杰[1] 王蕾[1] 孙会媛[1] 

机构地区：[1]秦皇岛出入境检验检疫局,河北秦皇岛066004

出　　处：《色谱》2013年第12期1201-1205,共5页Chinese Journal of Chromatography

基　　金：国家质检总局科技计划项目(2012IK155)

摘　　要：采用液相色谱-同位素比质谱(LC-IRMS)技术建立了同时测定葡萄酒中甘油和乙醇δ13C值的分析方法。优化了葡萄酒中影响甘油和乙醇色谱分离的条件。方法的精密度和准确度分别为0.15‰~0.26‰和0.11‰~0.28‰。对40个葡萄酒样品进行了测定,甘油和乙醇的δ13 C值分别为-26.87‰^-32.96‰、-24.06‰^-28.29‰,两者具有较强的相关性(R=0.82)。该方法不需要复杂的样品预处理,在相同条件下同时测定甘油和乙醇的δ13C值,较传统方法简单、快速。A novel procedure was established for the characterization of δ13C values of glycerol and ethanol in wine by liquid chromatography-isotope ratio mass spectrometry （LC-IRMS）. Several parameters influencing the separation of glycerol and ethanol from wine matrix were optimized. The precision and accuracy of the proposed method were 0. 15%0 to 0.26%o and 0. l 1%o to 0, 28%0, respectively. The results obtained for 40 wine samples displayed that the δ13C value of glycerol ranged from - 26.87%0 to - 32.96%0 and that of ethanol ranged from - 24.06%0 to - 28.29%0. Close correlations （R = 0.82） were obtained between the δ13C values of glycerol and ethanol. The proposed method didn＇ t need complex sample treatment, and the δ13C values of glycerol and ethanol in wine can be simultaneously determined, thus improving

关 键 词：液相色谱-同位素比质谱 δ13C值 甘油 乙醇 葡萄酒 

分 类 号：O658[理学—分析化学]