树突状细胞在食管癌T细胞抗肿瘤免疫中的作用  

The role of dendritic cells mediated antitumor immunity in esophageal carcinoma

作　　者：晏梅[1] 高冬玲[1] 文小岗[1] 付淑莉[1] 张云汉[1] 

机构地区：[1]河南医科大学第一附属医院病理科,河南医科大学病理教研室,河南郑州450052

出　　处：《中国癌症杂志》2000年第6期493-495,498,共4页China Oncology

基　　金：河南省自然科学基础研究项目 !( 9940 2 170 0 )

摘　　要：目的 :研究食管癌组织中树突状细胞 (DCs)在肿瘤浸润性T淋巴细胞激活中的作用。方法 :用免疫组化和RT PCR的方法 ,检测 46例食管癌手术标本中DCs(CD1a)、T细胞亚群 (CD3、CD4、CD8、CD2 5 )及共刺激因子B7。结果 :癌组织中的DCs与CD3(+)、CD4(+)T细胞有相关性 ,与CD8(+)、CD2 5 (+)T细胞无明显关系 ,且B7mRNA表达均为阴性。结论 :食管癌中DCs不一定能有效地激活T细胞 ,其中B7基因表达受抑制 ,可能是不能有效激活T淋巴细胞产生免疫耐受的重要原因之一。Purpose:To study the role of DCs in tumor infiltrating T lymphocytes activation of esophageal carcinomas.Methods:46 specimens of patients with esophageal carcinoma were used to investigate the DCs (CD1a), the subsets of tumor infiltrating T lymphoctyes (CD3,CD4,CD8, CD25) and costimulatory B7 using monoclonal antibodies by immunohistochemistry staining LSAB method and reverse transcription polymerase chain reaction (RT PCR). Results:There was significant correlation between CD1a(+) DCs and CD3(+),CD4(+) T lymphocytes, but no marked correlation between CD1a(+) DCs and CD8(+), CD25(+) T lymphocytes. No specific B7 mRNA expression was found in all specimens. Conclusions:DCs may not activate T lymphocytes efficiently. B7 gene was expressed at low level or deficiency in esophageal carcinoma may be one of the important reasons of failure to activate lymphocytes and induce the immunosuppression.

关 键 词：食管肿瘤 树突状细胞 T淋巴细胞 免疫耐受 

分 类 号：R735.1[医药卫生—肿瘤]