hEST2逆转录功能区片段与端粒酶活性相关性  

The correlation between hEST2 reverse transcription motif and the activity of telomerase

作　　者：朱涵能[1] 莫素珍[1] 罗伟华[1] 熊思东[2] 程文英[1] 

机构地区：[1]复旦大学医学院放射医学研究所,上海200032 [2]复旦大学医学院免疫学教研室,上海200032

出　　处：《中国癌症杂志》2000年第6期556-558,共3页China Oncology

基　　金：上海市科委重点资助项目! ( 9741190 40 1)

摘　　要：目的 :端粒酶是近年来发现的肿瘤重要的标志物 ,寻求端粒酶活性表达必需序列是研究端粒酶调控机制方法之一。方法 :本文通过RT PCR、Northernblot方法检测hEST2逆转录功能区片段表达 ,并以端粒酶活性检测hEST2逆转录功能区片段表达与端粒酶活性之间相关性 ,并以端粒酶活性TRAP法为对照。结果 :hESTα逆转录功能区片段与端粒酶活性相关性。结论 :含有包括端粒酶特异性T功能区片段在内的 7个逆转录功能区区域表达与端粒酶活性密切相关 。Purpose:Telomerase was discovered recently as an important tumor marker, and the necessary gene sequence which is closely related with telomerase activity is one of the methods to study the telomerase regulation mechanism. Methods:RT PCR, Northern blot were used to test the relationship between the expression of hEST2 reverse transcriptase motif fragment and telomerase activity, classic telomerase measurement method was set up as contol. Results:Indicate that the expression of 7 RT motifs fragment which include telomerase specific T motif have a close relationship with telomerase activity. Conclusions:It will be helpful to further research of telomerase regulation mechanism, diagnosis and inhibition.

关 键 词：端粒酶 hEST2 逆转录功能区 肿瘤 

分 类 号：R730.23[医药卫生—肿瘤]