Livin和细胞色素C在舌鳞状细胞癌组织中的表达及其临床意义  

Expressions of Livin and Cyt-C in tongue squamous cell carcinoma tissue and their clinical significances

作　　者：黄汉[1] 李方遒 张斌[1] 马竟[1] 

机构地区：[1]辽宁医学院附属第一医院口腔科,辽宁锦州121001 [2]辽宁医学院附属第一医院手术室,辽宁锦州121001

出　　处：《吉林大学学报（医学版）》2013年第6期1233-1236,1316,共5页Journal of Jilin University：Medicine Edition

基　　金：辽宁省教育厅高等学校科研项目资助课题(05L141)

摘　　要：目的:研究舌鳞状细胞癌(TSCC)组织中Livin和细胞色素C(Cyt-C)的表达,观察其表达水平与TSCC临床病理特征的关系,并探讨TSCC组织中Livin和Cyt-C蛋白表达的相关性。方法:采用免疫组织化学SP法检测48例TSCC和10例正常舌黏膜组织中Livin和Cyt-C蛋白的表达,并分析其与患者的性别、年龄、组织学类型和淋巴结转移等临床病理特征的相关性。结果:Livin主要表达在细胞浆中,TSCC和正常舌黏膜组织中Livin蛋白阳性表达率分别为66.67%和0%,二者比较差异有统计学意义(P<0.01);Livin的高表达与TSCC组织学分化程度有关联(P<0.05),并与颈淋巴结转移有关联(P<0.05)。Cyt-C主要表达在细胞浆中,TSCC和正常舌黏膜中Cyt-C蛋白阳性表达率分别为70.83%和10.00%,二者比较差异有统计学意义(P<0.01);Cyt-C蛋白阳性表达率与组织学分化程度有关联(P<0.05)。Spearman等级相关分析,TSCC组织中Livin和Cyt-C蛋白表达呈正相关关系(r=0.553,P<0.001)。结论:TSCC组织中Livin和Cyt-C蛋白表达呈正相关关系,二者的联合检测有助于临床判断TSCC恶性程度和评估预后。Objective To investigate the expressions of Livin and cytochrome C （Cyt-C） in tongue squamous cell Carcinoma （TSCC） tissue and to explore the correlation between their expression levels and the clinicaopathological characteristics of TSCC and the correlation between expressions of Livin and Cyt-C protein in TSCC tissue. Methods The expressions of Livin and Cyt-C protein were examined by immunohistochemistry SP method in 48 cases of TSCC and 10 cases of normal tongue tissue, and the relationship between the expressions of Livin, Cyt- C protein and the clinicopathological characteristics, such as gender, age, histological type and lymph node metastasis were analyzed. Results Livin mainly expressed in the cytoplasm. The positive expression rates of Livin protein in TSCC and normal tongue tissue were 66.67% and 0%, respectively, and there was significant difference （P〈0. 01）. The high expression of Livin was correlated to the histological differentiation degree of TSCC （P〈0.05）, and also closely correlated to the cervical lymph node metastasis （P〈0.05）. Cyt-C mainly expressed in the cytoplasm. The positive expression rates of Cyt-C protein in TSCC and normal tongue tissue were 70.83% and 10. 0%, respectively, and there was significant difference （P〈0.01）. The expression of Cyt-C was correlated to the histological differentiation of TSCC （P〈0.05）. The Spearman analysis results showed that there was a positive correlation between the expressions of Livin and Cyt-C protein in TSCC tissue （r=0.553, P〈0.001）. Conclusion There is a positive correlation between the expressions of Livin and Cyt-C protein in TSCC tissue. Combined detection of livin and Cyt-C will help to judge the malignant degree of TSCC and evaluate the prognosis.

关 键 词：舌鳞状细胞肿瘤 LIVIN 细胞色素C 

分 类 号：R739.86[医药卫生—肿瘤]