转录因子Oct-4和SOX-2在宫颈癌组织中的表达及其意义  

Expressions of transcription factor Oct-4and SOX-2 in cervical cancer tissue and their significances

作　　者：张璐[1] 魏振彤[1] 李佳佳[1] 郭慧宁[1] 张松灵[1] 

机构地区：[1]吉林大学第一医院肿瘤妇科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2013年第6期1252-1255,1316,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金青年基金资助课题(30901589);吉林大学白求恩B计划资助课题(20120027)

摘　　要：目的:研究转录因子Oct-4和SOX-2在宫颈癌及正常宫颈组织中的表达情况,阐明二者作为宫颈癌干细胞标记物的可能性。方法:采用免疫组织化学SP法检测Oct-4和SOX-2在52例宫颈癌组织和28例正常宫颈组织中的表达,分析二者在不同类型宫颈癌患者中的表达情况。结果:Oct-4在宫颈癌组织中表达率(46.1%,24/52)显著高于其在正常组织中的表达率(7.1%,2/28),二者比较差异有统计学意义(P<0.05);SOX-2在宫颈癌组织中的表达率(59.6%,31/52)显著高于其在正常组织中的表达率(10.7%,3/28),二者比较差异有统计学意义(P<0.05)。宫颈癌组织中Oct-4和SOX-2的表达与患者年龄、病理类型和FIGO分期均无关联性(P>0.05);与宫颈癌的分化程度有关联,随分化程度的降低,Oct-4和SOX-2表达水平升高,不同分化程度宫颈癌患者表达频数比较差异均有统计学意义(P<0.05);宫颈癌组织中Oct-4与SOX-2的表达频数呈正相关关系(r=0.753,P<0.05)。结论:Oct-4和SOX-2在宫颈癌组织中频数较高,且随宫颈癌分化程度降低表达频数升高,提示Oct-4和SOX-2可作为宫颈癌干细胞的标记物。Objective To investigate the expressions of transcription factor Oct-4 and SOX-2 in cervical cancer and normal cervix tissue and to explore the possibility of Oct-4 and SOX-2 as the stem cell markers of cervical cancer. Methods The expressions of Oct-4 and SOX-2 in 52 cases of cervical cancer tissue and 28 cases of normal cervix tissue were detected by immunohistochemical SP method. The expressions of Oct-4 and SOX-2 in the patients with cervical cancer with different types were analyzed. Results The expression of Oct-4 protein in cervical cancer tissue （46.1%, 24/52） was significantly higher than that in normal cervix tissue （7.1%, 2/28） （P〈0.05）; the expression of SOX-2 protein in cervical cancer tissue （59.6%, 31/52） was significantly higher than that in normal cervix tissue （10.7%, 3/28） （P%0.05）. The expressions of Oct-4 and SOX-2 in cervical cancer tissue were significantly correlated with the histological grade （P〈0.05） ; but not correlated with the age, pathological type, FIGO stage of the patients （P〈0. 05）. The expressions of OCt-4 amd SOX-2 were increased with the decreasing of the differentiation degree, and there were significant differences of the expression frequencies of OCt-4 and SOX-2 in the patients with different differentiation degrees （P〈0.05）. The expression of Oct-4 was positively related to the expression of SOX-2 in cervical cancer tissue （P〈0.05）. Oonclusion The expression frequencies of Oct-4 and SOX-2 in cervical cancer tissue are increased with the decreasing of differentiantion degree of cervical cancer which indicates that Oct-4 and SOX-2 may be used as the markers of cervical cancer stem cells.

关 键 词：OCT-4 sox-2 宫颈癌 免疫组织化学 肿瘤干细胞 

分 类 号：R737.33[医药卫生—肿瘤]