大粒车前子多糖组分PLP-1的结构表征  

Structural Characterization of Purified Polysaccharide Fraction( PLP-1) from the Seeds of Plantago asiatica L.

作　　者：殷军艺[1] 李昌[1] 聂少平[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047

出　　处：《高等学校化学学报》2013年第12期2728-2733,共6页Chemical Journal of Chinese Universities

基　　金：国家'八六三'计划项目(批准号:2013AA102102);'十二五'国家科技支撑计划项目(批准号:2012BAD33B06);国家自然科学基金重点项目(批准号:31130041);国家自然科学基金(批准号:21062012)资助

摘　　要：研究了由大粒车前子多糖分离纯化所得组分PLP-1的一级精细结构特征.通过单糖组成分析、部分酸水解及甲基化分析,并结合NMR,GC和GC-MS等技术测定了PLP-1的一级结构.结果表明,PLP-1由Ara(33.98%),Xyl(58.23%),Rha(2.36%),Glc(2.23%),Gal(2.36%)和Man(0.83%)组成,糖醛酸以GlcA形式存在.PLP-1中主要糖残基有α-T-linked Araf(9.58%),β-T-linked Xylp(8.71%),α-1,3-linked Araf(18.22%),β-1,3-linked Xylp(8.05%),β-1,4-linked Xylp(5.85%),β-1,2,4-linked Xylp(16.98%)和β-1,3,4-linked Xylp(27.52%),GlcA以α-T-linked GlcAp形式存在;此外还有1,2-linked Rhap,T-linked Glcp,1,6-linked Glcp,T-linked Galp,1,3-linked Galp和1,3,6-linked Galp等少量其它残基.根据所得结果推断出PLP-1可能的一级结构.Polysaccharide＇ s biological activities are related to their structure. Previous studies showed that the polysaccharide from seeds of Plantago asiatica L. had good bioactivities, and two fractions from the crude polysaccharide were characterized. This work aimed at investigating primary structure of another fraction, PLP-1. The methods, including monosaccharide compositions analysis, partial acid hydrolysis, nuclear mag- netic resonance （ NMR ）, gas chromatography （ GC ） and gas chromatography-mass spectroscopy （ GC-MS ）, were applied for structural characterization. The results showed that PLP-1 Was composed of Ara（33.98% ）, Xyl（58.23% ）, Rha（2.36% ）, Glc（2. 23% ）, Gal（2. 36% ） and Man（0. 83% ）. Its main uronic acid was GlcA. The majority residues were a-T-linked Araf（9.58% ）,/3-T-linked Xylp（ 8.71% ）, a-1,3-1inked Araf （18.22%）, E-1,3-1inked Xylp （8.05%）, E-I, 4-1inked Xylp （5.85 % ）, E-l, 2,4-1inked Xylp（ 16. 98% ） and/3-1,3,4-1inked Xylp（27.52% ）. GlcA was existed as a-T-linked GlcAp. There were other residues, in- cluding 1,2-1inked Rhap, T-linked Glcp, 1,6-1inked Glcp, T-linked Galp, 1,3-1inked Galp and 1,3,6-1inked Galp, etc. Finally, primary structure of PLP-1 was proposed.

关 键 词：大粒车前子 多糖 一级结构 甲基化 

分 类 号：O629.12[理学—有机化学]