HPLC法同时测定乐脉胶囊中9种成分  

Simultaneous determination of nine components in Lemai Capsules by HPLC

作　　者：董娟妮[1] 闫高颖 杨洋[1] 王世祥[1] 郑静[1] 刘勤社[1,2] 郑晓晖[1,3] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]陕西省人民医院,陕西西安710068 [3]深圳清华大学研究院,广东深圳518057

出　　处：《中成药》2013年第12期2644-2649,共6页Chinese Traditional Patent Medicine

基　　金：国家科技支撑计划课题(2008BAI51B01);陕西省自然科学基金项目(2010JM4047;2011JM4042);陕西省中医药管理局项目(ZY47);西北大学大学生创新基金(2011007)

摘　　要：目的建立HPLC法同时测定乐脉胶囊（丹参、川芎、赤芍、红花、香附等）中丹参索、丹参酮ⅡA、原儿茶醛、原儿茶酸、阿魏酸、没食子酸、芍药苷、羟基红花黄色素A和d一香附酮9种有效成分的量。方法采用AgilentTC—C18色谱柱（4．6mm×250mm，5μm）；以0．2％甲酸水．甲醇为流动相梯度洗脱；体积流量0．6mlfmin；柱温30℃；检测波长280nm（0—20min），403nm（20一22min），230nm（22～26rain），320nm（26～45rain）和254nm（45—60min）。结果丹参素在5．0～500．0μ∥mL，丹参酮ⅡA和d一香附酮在1．0—100．0仙g／mL，原儿茶酸和原儿茶醛在2．5—250．0Ixg／mL，阿魏酸在1．7～170．0斗∥mL，没食子酸在10．0～1000．0μ/mL，芍药苷在20．0～2000．0μg／mL，羟基红花黄色素A在4．6～460．0p∥mL范围内线性关系良好（r≥0．9990），平均加样回收率均大于95．0％。结论本方法简便、准确、重复性好，可作为乐脉胶囊的质量控制方法。AIM To establish an HPLC method for simultaneously determining nine components in Lemai Cap- sules （Salviae miltiorrhizae Radix et Rhizome, Chuanxioag Rhizome, Paeoaiae Radix rubra, Carthami Flos, Cyperi Rhizoma）, including danshensu, tanshinone II A, protocatechuic aldehyde, protocatechuic acid, ferulic acid, gal- lic acid, peoniflorin, hydroxysafflor yellow A and ot-cyperone. METHODS The analysis was carried out on Agi- lent TC-Cls column （4. 6 mmx 250 ram, 5 iμm）. The mobile phase consisted of methanol-0. 2% formic acid-wa- ter with step gradient elution. The flow rate was 0.6 mL/min, the column temperature was 30 C and the detection wavelengths were 230 rim, 254 nm, 280 nm, 320 nm, and 403 nm respectively. RESULTS The samples showed a good relationship in the ranges of 5.0 500. 0 Ixg/mL for danshensu, 1.0 100.0 μg/mL for tanshinone II A and c-cyperone, 2.5 -250. 0 ixg/mL for protocatechuic acid and protocatechuic aldehyde, 1.7 - 170. 0 Ixg/mL for fer- ulic acid, 10.0 1 000.0 μg/mL for gallic acid, 20.0 - 2 000.0 μg/mL for peoniflorin and 4.6 460 g/mL for hydroxysafflor yellow A, respectively （ r I〉 0. 999 0）. The average recoveries of the nine compoments were more than 95.0%. CONCLUSION This method with strong specificity is simple, accurate, reproducible and can be used for quality control of Lemai Capsules.KEY WORDS ：

关 键 词：乐脉胶囊 丹参素 丹参酮ⅡA 阿魏酸 羟基红花黄色素A d 香附酮 

分 类 号：R927.2[医药卫生—药学]