硫酸水解蚕丝蛋白制备丝素肽的研究  

Preparation of Silk Fibroin Peptide by Sulfuric Acid Silk Protein

作　　者：曹新志[1] 李琳琳[1] 熊俐[1] 赵迎庆[1] 任林生[1] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000

出　　处：《安徽农业科学》2013年第26期10828-10831,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]获得制备丝素肽的简便高效的方法。[方法]以废蚕丝为原料,用0.5%碳酸钠水溶液进行脱胶,硫酸进行水解,氧化钙水溶液中和沉淀,活性炭脱色制备丝素肽;采用正交试验L9(34)优化硫酸水解工艺条件。[结果]试验表明,蚕丝蛋白在固液比1∶40 g/ml,硫酸浓度30%,水解温度80℃,水解时间9 h的条件下进行水解时,丝素肽的得率最高。[结论]研究可为丝素肽的进一步工业化生产提供可靠的理论依据。[Objective] To obtain simple and high efficient method for preparation of silk fibroin peptide.[Method] With waste silk as raw materials,silk fibroin peptide was prepared by refining waste silk with 0.5％ sodium carbonate solution,hydrolyzing silk fibroin with sulfuric acid,desalting with precipitation separation method,decolorizing with activated carbon.Hydrolysis process condition was optimized by using orthogonal test L9 （34）.[Result] The results showed that silk fibroin peptide yield was the highest when silk protein was hydrolyzed under the conditions of solid-liquid ratio of 1∶ 40,the sulfuric acid concentration of 40％,hydrolysis temperature of 80 ℃,hydrolysis time 9 h.[Conclusion] This study provided reliable theoretical basis for the industrial production of silk fibroin peptide.

关 键 词：硫酸水解 正交试验 丝素肽 

分 类 号：S509.9[农业科学—作物学]