不同前列腺癌细胞对成骨前体细胞增殖和成熟能力的影响  

Effects of different prostate cancer cells on the proliferation and differentiation of osteoblastic progenitor cells

作　　者：刘复州[1] 邱浩[1] 王汝杰[1] 邹传奇 刘栓得[1] 胡旭[1] 沈伟伟[1] 张超[1] 周跃[1] 初同伟[1] 

机构地区：[1]第三军医大学第二附属医院骨科,重庆400037

出　　处：《肿瘤》2013年第12期1033-1038,共6页Tumor

基　　金：国家自然科学基金面上项目资助(编号:81271979)

摘　　要：目的 :探讨前列腺癌LNCaP和PC-3细胞条件培养液(conditioned medium,CM)对成骨前体细胞MC3T3-E1增殖和分化的影响。方法 :分别用含20%LNCaP CM、20%PC-3 CM和不含CM的α-MEM培养液(作为对照组)处理MC3T3-E1细胞。CCK-8法检测各组细胞的增殖情况,碱性磷酸酶(alkaline phosphatase,ALP)活性检测试剂盒检测各组细胞中ALP的活性,茜素红染色法检测各组细胞的钙化情况,实时荧光定量-PCR法检测各组细胞中Ⅰ型胶原蛋白α1(collagen typeⅠalpha 1,Col1α1)、骨钙蛋白(osteocalcin,OCN)和RUNX2(runt-related transcription factor 2)的mRNA表达水平。结果 :与对照组和LNCaP CM组相比,PC-3CM组MC3T3-E1细胞的增殖能力明显增高(P<0.05);LNCaP CM组MC3T3-E1细胞中ALP的活性明显高于对照组(P<0.05),钙化面积大于对照组(P<0.05);PC-3 CM组MC3T3-E1细胞中ALP的活性较对照组明显降低(P<0.05),钙化面积明显小于对照组(P<0.05);LNCaP CM组MC3T3-E1细胞中Col1α1、OCN和RUNX2的mRNA表达水平均明显高于对照组(P<0.05),而PC-3 CM组MC3T3-E1细胞中Col1α1、OCN和RUNX2的mRNA表达水平均明显低于对照组(P<0.05)。结论 :PC-3 CM可明显促进成骨前体细胞增殖,但抑制其分化成熟;而LNCaP CM可显著促进成骨前体细胞分化成熟,说明该模型能够充分反映不同前列腺癌细胞对成骨前体细胞增殖和分化的差异,可以作为后续研究前列腺癌骨转移机制的体外模型。Objective： To investigate the effects of conditioned medium （CM） of prostate cancer LNCaP and PC-3 cells on the proliferation and differentiation of pre-osteoblastic cell MC3T3-E1. Methods： MC3T3-E1 cells were incubated with 20% LNCaP CM, 20% PC-3 CM or α-MEM without CM （as a control）, respectively. The proliferation of MC3T3-E1 cells was detected by cell counting kit-8 （CCK-8） method. The alkaline phosphatase （ALP） assay kit was used to evaluate the ALP activity in MC3T3-E1 cells. The alizarin red staining was used to detect the mineralization of MC3T3-E1 cells. The mRNA expression levels of collagen type I alpha 1 （Co11α1）, osteocalcin （OCN） and runt-related transcription factor 2 （RUNX2） mRNAs were measured by real-time fluorescence quantitative-PCR. Results： As compared with the control and LNCaP CM groups, PC-3 CM promoted the proliferation of MC3T3-E1 cells notably （P 〈 0.05）. The ALP activity in MC3T3-E1 cells in the LNCaP CM group was higher than that in the control group （P 〈 0.05）, and the area of mineralizaion nodes was wider （P 〈 0.05）, but the result was opposite in the PC-3 CM group. The expression levels of Co11α1, OCN and RUNX2 mRNAs in MC3T3-E1 cells in LNCaP CM group were higher than those in the control group （P 〈 0.05）, but PC-3 CM inhibited the expression levels of these mRNAs （P 〈 0.05）. Conclusion： The PC-3 CM can stimulate the proliferation of osteoblastic progenitor cells and inhibit their differentiation. On the contrary, LNCaP CM can promote the differentiation of osteoblastic progenitor cells. These models can be used in vitro for further researchon prostate cancer bone metastasis, as they can affect proliferation and differentiation of osteoblastic progenitor cells differently.

关 键 词：前列腺肿瘤 细胞增殖 细胞分化 体外研究 成骨前体细胞 

分 类 号：R737.25[医药卫生—肿瘤]