^125Ⅰ粒子短时低剂量率照射对胰腺癌Capan-2细胞神经浸润的影响  

Effect of Iodine 125 seeds short time low dose rate irradiation on perineural invasion in pancreatic cancer Capan-2 cells

作　　者：司佩任[1] 路筝[2] 刘岩[2] 马建霞[2] 吴洪玉[2] 李兆申[2] 

机构地区：[1]解放军107医院,山东烟台264002 [2]第二军医大学长海医院消化内科

出　　处：《中华胰腺病杂志》2013年第6期361-365,共5页Chinese Journal of Pancreatology

基　　金：国家自然科学青年基金（30801362）

摘　　要：目的 观察^125I粒子短时低剂量率照射对胰腺癌Capan-2细胞神经浸润的影响,并探讨其分子机制.方法 建立胰腺癌Capan-2细胞和大鼠背根神经节（DRG）共培养及Capan-2或DRG单培养模型.通过125I粒子低剂量率照射平板对3种模型进行照射,以相应未照射模型作为对照.倒置显微镜下观察癌细胞、DRG的生长,图像分析软件计算神经突和癌细胞集落占据的表面积,ELISA法检测细胞培养上清液和基质胶溶解液中神经生长因子（NGF）及转化生长因子α（TGF-α）浓度,RT-PCR法检测胰腺癌Capan-2细胞神经营养因子-3（NT-3）mRNA表达.结果 共培养模型中DRG发出的神经突向癌细胞定向、集中生长,而癌细胞沿神经突的方向生长.经125I粒子照射后这种定向、集中和互逆的生长受到一定程度的抑制.共培养组第5天所增加的神经突表面积为290.15±12.08,较DRG单培养组的124.83±6.96显著增加（P＜0.01）,经照射后的神经突表面积减少到201.53±12.20（P ＜0.01）;所增加的Capan-2细胞表面积为300.47±12.99,较Capan-2细胞单培养组的199.30±8.60显著增加（P＜0.01）,经照射后的Capan-2细胞表面积减少到202.35±7.97 （P ＜0.01）.共培养组不表达NT-3mRNA,经照射后NT-3mRNA表达量为0.68±0.04（P ＜0.05）.共培养组培养上清液中NGF及TGF-α浓度分别为（27.56 ±13.73）、（40.86±20.73） ng/ml,经照射后分别升高到（94.98±33.80）、（157.54±83.76） ng/ml,差异有统计学意义（P＜0.05或＜0.01）.共培养组基质胶溶解液中NGF及TGF-α浓度分别为（60.42 ±33.03）、（64.39±21.52） ng/ml,经照射后分别升高到（132.52±53.01）、（138.38±83.58） ng/ml,其中NGF的差异有统计学意义（P＜0.05）.结论 125I粒子短时低剂量率照射可以抑制胰腺癌和神经的交互作用,其机制可能与癌细胞促神经浸润介质NGF、TGF-α和NT-3等表达上调有关.Objective To investigate the effect of Iodine 125 seeds short time low dose rate irradiation on perineural invasion （PNI） in pancreatic cancer Capan-2 cells,and explore its molecular mechanism.Methods The co-culture model was established by co-culturing the dorsal root ganglion （DRG） of SD rat and Capzn-2 cells line,while Capan-2 culture model and DRG culture model was also established.Iodine 125 seeds short time low dose rate irradiation tablet was used for the 3 models,and the model without irradiation was used as control.Cancer cell and DRG growth was observed under inverted microscopy,surface of neurite and cell colony growth was determined by image analysis software.The concentration of nerve growth factor （NGF）,transforming growth factor-α （TGF-α） in cell culture supernatant and matrigel solution was tested by ELISA,and the expression of neurotrophin-3 （NT-3） mRNA was detected by semi-quantitative reverse transcriptase polymerase chain reaction （RT-PCR）.Results In the co-culture model,neurite of DRG showed a direction to cancer cells and had a concentrated growth towards cancer cells.And Capan-2 cells formed more colonies towards neurite.However,in irradiation groups,the symbiotic phenomenon was inhibited to some degree.Increased surface of neurite in co-culture model at 5th day was 290.15 ± 12.08,which was significantly higher than that in DRG group （124.83 ± 6.96,P 〈 0.01）,but the surface of neurite was decreased to 201.53 ± 12.20 after irradiation （P 〈0.01）.Increased surface of Capan-2 cell was 300.47 ± 12.99,which was significantly higher than that in Capan-2 group （199.30 ± 8.60,P 〈 0.01）,but the surface of Capan-2 was decreased to 202.35 ± 7.97 after irradiation （P 〈 0.01）.NT-3 mRNA was seldom or not expressed in supernatant of co-culture model,but it was strongly expressed （0.68 ± 0.04） after irradiation （P 〈 0.05）.The concentration of NGF and TGF-α in supernatant of co-culture model were （27.56 ± 13.73）,（40.86 ± 20.73） ng/

关 键 词：胰腺肿瘤 碘同位素 小剂量照射 肿瘤浸润 

分 类 号：R735.9[医药卫生—肿瘤]