人参皂苷Rb1对SweAPP-SK细胞保护作用的研究  

Protective effects of Ginsenoside Rb1 on SweAPP-SK cells

作　　者：杨玲玲[1] 孙高英[2] 郝建荣[2] 魏玉平[2] 郭元芳[2] 崔行[2] 毕文祥[2] 

机构地区：[1]北京市红十字血液中心,北京100088 [2]山东大学医学院生物化学与分子生物学研究所

出　　处：《中国老年学杂志》2013年第24期6187-6189,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金青年科学基金(No.8000146);山东省青年科学家科研奖励基金(No.BS2010YY035)

摘　　要：目的观察人参皂苷Rb1对SweAPP-SK细胞的保护作用。方法用Rb1处理SweAPP-SK细胞,用MTT分析、酶联免疫分析和流式细胞术观察Rb1对SweAPP-SK细胞增殖、Aβ分泌量、细胞活性氧(ROS)水平和Ca2+水平的影响。结果较SK-N-SH细胞,SweAPP-SK细胞增殖明显延迟,细胞ROS及Ca2+水平增加。用Rb1处理SweAPP-SK细胞后,细胞增殖加快,细胞外Aβ40浓度明显降低,细胞ROS及Ca2+水平明显降低。结论 Rb1对SweAPP-SK细胞有保护作用,该保护作用的发挥可能与其能减少Aβ分泌及降低细胞内ROS和Ca2+水平有关。Objective To explore the protective effects of Ginsenoside Rbl on SweAPP-SK cells, which were constructed using human neuroblastoma SK-N-SH cells, had a high expression level of amyloid precursor protein （APP） and secreted more β-amylold peptide （ Aβ）. Methods SweAPP-SK cells were treated with Rbl, and MTT assay, ELISA and flow cytometry were used to examine cell proliferation, Aβ secretion, and intracellular reactive oxygen species （ROS） and Ca2＋ levels. Results SweAPP-SK cells proliferated slower and had higher levels of ROS and Ca2＋ compared with SK-N-SH cells. After treated with Rbl, SweAPP-SK cells showed a faster proliferation, secreted less Aβ, and had lower levels of ROS and Ca2＋ in comparison with the untreated. Conclusions Rbl has neuroprotective effects, which maybe associate with its abilities to decrease Aβ secretion by neurocytes and levels of ROS and Ca2＋ in them.

关 键 词：阿尔茨海默病 Β-淀粉样肽 人参皂苷RB1 活性氧 CA2+ 

分 类 号：R741.02[医药卫生—神经病学与精神病学]