糖尿病脂肪肝患者网膜素-1与脂联素、炎症因子的关系  

Association of Plasma Omentin-1 Levels with Adiponectin and Inflammatory Cytokines in Diabetic Patients with Fatty Liver

作　　者：邵金双 贾国瑜[2] 王璐[2] 李强[2] 张洁[2] 刘玉清[1] 邸阜生[2] 

机构地区：[1]天津医科大学三中心临床学院,300170 [2]天津市第三中心医院内分泌科,天津市人工细胞重点实验室

出　　处：《天津医药》2013年第12期1169-1172,共4页Tianjin Medical Journal

基　　金：天津市卫生局科技基金重点资助项目(项目编号:2010KR02)

摘　　要：目的研究2型糖尿病(T2DM)合并非酒精性脂肪性肝病(NAFLD)患者血浆网膜素-1与脂联素和肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、高敏C反应蛋白(hs-CRP)等炎症因子的关系。方法采用酶联免疫吸附法(ELISA)检测T2DM合并NAFLD组(A组,63例)、T2DM不合并NAFLD组(B组,63例)和正常对照组(C组,70例)的血浆网膜素-1和脂联素水平,同时测定上述3组生化指标及炎症指标(hs-CRP、TNF-α和IL-6),应用相关分析和多元回归分析方法分析血浆网膜素-1与脂联素及炎症指标的关系,应用Logistic回归分析T2DM合并NAFLD的影响因素。结果A组血浆网膜素-1(27.02±2.82)μg/L、脂联素水平(11.98±3.63)mg/L低于B组[分别为(31.52±2.81)μg/L、(15.85±3.28)mg/L]和C组[分别为(35.92±2.80)μg/L、(19.88±3.44)mg/L],且B组低于C组(P<0.01),A组血浆网膜素-1水平与脂联素、高密度脂蛋白胆固醇(HDL-C)正相关,与TNF-α、IL-6、空腹血糖(FBG)、胰岛素抵抗指数(HOMAIR)、内脏脂肪面积、腰围、腰臀比(WHR)、游离脂肪酸(FFA)负相关(P<0.05或P<0.01);多元逐步线性回归分析表明脂联素、TNF-α、IL-6是血浆网膜素-1水平的影响因素。Logistic回归分析显示网膜素-1、hs-CRP、内脏脂肪面积及游离脂肪酸(FFA)是T2DM合并NAFLD的独立影响因素(P<0.01)。结论T2DM患者合并NAFLD与网膜素-1降低相关,网膜素-1表达可能会受到脂联素及炎症因子的影响。Objective To investigate the relationship of omentin-1 with adiponectin and inflammatory cytokines in type 2 diabetes （T2DM） patients with nonalcoholic fatty liver disease （NAFLD）. Methods The serum levels of omentin-1 and adiponectin were assayed by enzyme-linked immunosorbent assay （ELISA） in patients of T2DM with NAFLD （group A, n=63）, T2DM without NAFLD （group B, n=63）and normal control group （group C, n=70）. At the same time the biochemical markers and inflammatory marker, such as tumor necrosis factor （TNF）-α, high-sensitivity C-reactive protein （hs-CRP） and interleukin 6（IL-6） were detected in three groups. The correlation analysis and multiple regression analysis were used to de-tect the association of omentin-1 with adiponectin and inflammatory markers. The logistic regression was used to analyze fac-tors influencing NAFLD in patients with T2DM. Results The serum levels of omentin-1 and adiponectin were significant-ly lower in group A [ （27.02±2.82）μg/L and （11.98±3.63） mg/L] than those of group B [（31.52±2.81）μg/L and （15.85±3.28） mg/L] and group C [（35.92±2.80）μg/L and （19.88±3.44） mg/L], and there were significantly lower levels of them in group B than those of group C （P〈0.01）. The plasma omentin-1 level was positively correlated with adiponectin and high density li-poprotein （HDL-C） in group A. Also the plasma omentin-1 level was negatively correlated with TNF-α, IL-6, fasting blood glucose （FBG）, homeostasis model assessment of insulin resistance （HOMA-IR）, visceral adipose tissue, waist, waist-to-hip ratio （WHR） and free fatty acid in group A （P〈0.05 or P〈0.01）. Multiple stepwise regression analysis showed that adipo-nectin, TNF-αand IL-6 were independent factors influencing the level of plasma omentin-1. Logistic regression analysis showed that omentin-1 was one of independent factors influencing T2DM combined with NAFLD （P〈0.01）. Conclusion The incident of NAFLD in T2DM patients is related

关 键 词：糖尿病 2型 脂肪肝 脂联素 炎症 脂肪因子类 网膜素-1 

分 类 号：R575.5[医药卫生—消化系统]