MCPIP1介导MCP-1诱导的血管平滑肌细胞增殖  

MCPIP1 mediates MCP-1-induced vascular smooth muscle cell proliferation

作　　者：杨一峻[1] 吴柳松[1] 束波[1] 钱民章[1] 

机构地区：[1]遵义医学院生物化学教研室,遵义563003

出　　处：《生理学报》2013年第6期616-622,共7页Acta Physiologica Sinica

基　　金：supported by the Science Foundation of Zunyi Medical College;China(No.F-613)

摘　　要：本文旨在探讨单核细胞趋化蛋白-1诱导蛋白1(monocyte chemotactic protein-1 induced protein-1,MCPIP1)是否介导单核细胞趋化蛋白-1(monocyte chemotactic protein-1,MCP-1)诱导的血管平滑肌细胞(vascular smooth muscle cell,VSMC)增殖。用Real-time PCR及Western blot检测体外培养的VSMC中MCP-1诱导的MCPIP1表达;用siRNA沉默VSMC中MCPIP1基因后,用细胞计数板记录细胞数量,用CCK-8试剂盒检测细胞活力,用EdU试剂盒检测DNA合成率,用流式细胞术检测S期细胞数量,用Real-time PCR检测MCP-1或血小板衍生生长因子(platelet-derived growth factor,PDGF)诱导的c-fos mRNA水平变化。结果显示,MCP-1提高VSMC中MCPIP1 mRNA水平,并呈剂量和时间依赖性地上调MCPIP1蛋白表达。和MCP-1处理的VSMC相比,MCPIP1基因沉默后VSMC的细胞数量、细胞活力及细胞DNA合成率均明显降低,S期细胞数量下降;MCPIP1基因沉默可抑制MCP-1或PDGF对c-fos mRNA的上调作用。以上结果提示,MCPIP1介导了MCP-1诱导的VSMC增殖,其机制涉及对c-fos表达的上调。The aim of the present study is to investigate whether monocyte chemotactic protein-1 （MCP-1）-induced vascular smooth muscle cell （VSMC） proliferation is mediated via monocyte chemotactic protein-1 induced protein-1 （MCPIP 1）. MCPIP l expressions in cultured VSMC were detected by real-time PCR and Western blot following MCP-1 incubation. After MCPIP1 was silenced by siRNA, cell number was counted by hemocytometer, VSMC activity was analyzed by CCK-8 kit, percentage of DNA synthesis was detected by EdU kit, percentage of S phase cell numbers were measured by flow cytometry, and c-los mRNA expression induced by MCP-1 or platelet-derived growth factor （PDGF） was detected by real-time PCR. The results showed MCP-1 increased MCPIP1 mRNA and up-regulated MCPIP1 protein expression in dose- and time-dependent manners. Cell counts, cellular activity, the percent- age of DNA synthesis, and the percentage of S phase cell numbers were remarkably decreased in MCPIP1 siRNA group, compared with those in MCP-1 group. The enhancing effect of MCP-1 or PDGF on c-fos mRNA expression was inhibited by MCPIP1 siRNA. These results suggest that MCP-l-induced VSMC proliferation is mediated via MCPIP1, and the underlying mechanism may involve c-fos expression up-regulation.

关 键 词：单核细胞趋化蛋白-1 单核细胞趋化蛋白-1诱导蛋白1 平滑肌细胞增殖 C-FOS基因 

分 类 号：R730.23[医药卫生—肿瘤]