枸杞多糖和原花青素对小鼠成釉细胞DNA损伤作用的研究  

Effects of lycium barbarum polysaccharides and procyanidins on DNA damage in mouse ameloblasts

作　　者：贺凌飞[1] 周小燕[1] 陈慧芝[1] 韩海燕[1] 陈秉[2] 余日安[2] 

机构地区：[1]广东药学院附属第一医院口腔科,广东广州510080 [2]广东药学院公共卫生学院劳动卫生与环境卫生学系,广东广州510310

出　　处：《临床口腔医学杂志》2013年第12期707-709,共3页Journal of Clinical Stomatology

基　　金：广东省医学科研基金资助项目(A2008331)

摘　　要：目的：探讨枸杞多糖和原花青素对小鼠成釉细胞DNA损伤的保护作用。方法：小鼠成釉细胞以100．00、200．00、400．00mg／L枸杞多糖作为枸杞多糖作用组，以10．00、25．00、50．00mg／L原花青素作为原花青素作用组。细胞染毒24h后，采用单细胞凝胶电泳技术（SCGE）检测DNA单链断裂情况。结果：与对照组比较，100．00、200．00和400．00mg／L枸杞多糖作用组小鼠成釉细胞尾长、Olive尾距和尾长／头长值均明显下降，差异具有统计学意义（P〈0．05）。100．00、400．00mg／L枸杞多糖作用组尾DNA％较对照组明显下降（P〈0．05）。与对照组比较，10．0HD、25．00、50．00mg／L原花青素作用组小鼠成釉细胞DNA损伤的尾长、Olive尾矩、尾DNA％、尾长／头长值均较对照组均显著性下降，差异具有统计学意义∽〈0．05）。以Olive尾矩为观察指标，100．00mg／L枸杞多糖作用组的Olive尾矩明显低于200．00mg／L和400．00mg／L枸杞多糖作用组（尸〈0．05），10．00mg／L原花青素作用组的Olive尾矩明显低于25．00mg／L和50．00mg／L原花青素作用组（尸〈0．05）。结论：100．00～400．00mg／L枸杞多糖和10．00～50．00mg／L原花青素对小鼠成釉细胞DNA损伤具有较好的保护作用，以100．00mg／L枸杞多糖、10．00mg／L原花青素的保护作用相对较好。Objective： To investigate the protective effects of Lycium barbarum polysaccharides （LBP） and Procyani- dins （PC） on DNA damage in mouse ameloblasts. Method： The mouse ameloblasts were treated with LBP at the doses of 100.00,200.00 and 400.00 mg / L, and PC at the doses of 10.00, 25.00 and 50.00 mg / L respectively. After exposed 24 h, the single cell gel electrophoresis assay was used to measure the DNA damage in mouse ameloblasts. Result： The tail length, olive tail moment and tail / head length ratio of LBP group at the doses of 100.00, 200.00 and 400.00 mg / L were lower than those of the control group in mouse ameloblasts （P 〈0.05）. LBP at the doses of 100.00 and 400.00 mg / L could de- crease DNA % compared with control （P 〈0.05）. PC at the doses of 10.00, 25.00 and 50.00 mg / L decreased the tail length, olive tail moment, DNA % and tail/head length ratio in mouse ameloblasts compared with control group （P 〈0.05）. Selected o- live tail moment as effective indicator, the protective effects of LBP at the dose of 100.00 mg / L were better than those of 200.00 and 400.00 mg / L LBP, and also 10.00 mg / L PC better than those of 25.00 and 50.00 mg / L PC. Conclusion： It was suggested that Lycium barbarum polysaccharides at the dose of 100.00-400.00 mg / L and Procyanidins at the dose of 10.00NS0.00 mg / L could inhibit DNA damage in mouse ameloblasts respectively, 100.00 mg/ L Lycium barbarum polysac- charides and 10.00 mg / L Procyanidins have relative better protective effect.

关 键 词：枸杞多糖 原花青素 成釉细胞 DNA损伤 

分 类 号：R780.2[医药卫生—口腔医学]