调节性T细胞和Th17细胞在OLP中的表达  

Expression of regulatory T cells and Helper T cells 17 in the lesion tissue of oral lichen planus

作　　者：白杨[1] 王文梅[1] 王翔[1] 段宁[1] 蒋红柳[1] 

机构地区：[1]南京大学口腔医学院.南京市口腔医院,江苏南京210008

出　　处：《临床口腔医学杂志》2013年第12期712-715,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金项目(81070839);南京市医学科技发展重点项目(zkx10530)

摘　　要：目的:通过观察调节性T细胞(regulatory T cells,Treg)和Th17细胞(helper T cells 17,Th17)在口腔扁平苔藓(OLP)组织中的表达情况,探讨其在OLP发生发展过程中的变化。方法:应用免疫组织化学双标记技术检测35例OLP患者和19例对照组口腔黏膜组织中CD25+Foxp3+、CD4+IL-17+细胞的表达。结果:OLP组病损组织中有大量CD25+Foxp3+细胞浸润,较对照组显著增多,两者差异非常显著(P<0.01)。CD4+IL-17+细胞在OLP组织中的表达较正常口腔黏膜组织有增加趋势,但差异无统计学意义(P>0.05)。经进一步统计学分析,CD25+Foxp3+细胞和CD4+IL-17+细胞数量改变在OLP组织中表达呈正相关(P<0.05)。结论:Treg细胞的数量在OLP组织中增多,Th17细胞可能协同Treg细胞,在OLP发生发展过程中发挥了一定的作用。Objective：The study was purposed to explore the changes of regulatory T cells and helper T cells 17 in le- sions tissue of patients with oral lichen planus, nethod：Immunohistochemistry double stainings（CD25＋ Foxp3＋ and CD4＋ IL- l7＋） were performed on tissue sections of 35 OLP,compared with 19 normal oral mucosa tissues. Result：OLP biopsy samples provide evidence for a strong infiltration of CD25＋ Foxp3~ Treg cell,compared with control group （P 〈0.01）. Also in lesions of OLP patients,the frequency of CD4＋ IL-17＋ T cells increased,compared with in normal oral mucosa,but no statistical signifi- cance was found （P 〉0.05）. In addition,positive correlation was shown between the density of CD25＋ Foxp3＋ T cells and CD4＋ IL-17＋ T ceils in OLP （P 〈0.05）. Conclusion：A significantly higher frequency of CD25＋ Foxp3＋ Treg cells could be observed in OLP group, In assistance with Thl7 cells,Treg cells may play a certain role during the development of OLP.

关 键 词：调节性T细胞 TH17细胞 口腔扁平苔藓 免疫组织化学 双标记染色 

分 类 号：Q786[生物学—分子生物学] R781.5[医药卫生—口腔医学]