番茄红素对慢性锰染毒大鼠学习记忆的改善作用  

Effect of lycopene on learning and memory in rats exposed to manganese chloride

作　　者：刘重斌[1,2] 陆建峰[1] 郭贺杰 陈露[1] 王瑞[1] 

机构地区：[1]湖州师范学院医学院生理与药理学教研室,浙江湖州313000 [2]温州医科大学基础医学院生理学教研室,浙江温州325035

出　　处：《中国药理学与毒理学杂志》2013年第6期948-953,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：浙江省自然科学基金(Y2110388);浙江省科技厅公益性应用研究项目(Y2011C37091);湖州师范学院校级实验室开放项目(Y20110330)~~

摘　　要：目的探讨番茄红素对慢性锰染毒大鼠学习记忆改善作用的机制。方法雄性SD大鼠ip MnCl2·4H2O 15 mg·kg-1,同时ig给予番茄红素5,10和15 mg·kg-1,每天1次,连续3个月。分别于第1,2和3个月末进行水迷路实验测定大鼠逃避潜伏期和目标象限游泳时间。实验结束后,取血、肝和海马组织,原子吸收光谱法测定血、肝和海马组织中锰含量。化学比色法检测肝和海马组织中丙二醛(MDA)含量及总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)酶活性。结果与正常对照和番茄红素组相比,慢性锰染毒组大鼠平均逃避潜伏期和目标象限游泳时间显著增加(P<0.05);血、肝和海马组织中锰含量显著升高(P<0.05);肝和海马组织中MDA含量显著增加(P<0.05),SOD,GSH-Px和CAT酶活性显著下降(P<0.05)。给予番茄红素15 mg·kg-1干预后,大鼠平均逃避潜伏期在第1,2和3个月末分别由慢性锰染毒组的50.7±10.8,46.1±9.2和(51.4±13.6)s显著降至42.1±4.0,27.5±4.6和(26.0±4.7)s(P<0.05);目标象限游泳时间在第2和3个月末分别由慢性锰染毒组的38.7±9.2和(45.2±14.2)s显著降至26.2±4.8和(27.3±8.3)s(P<0.01);血、肝和海马组织中锰含量显著下降(P<0.05);肝和海马组织中MDA含量显著降低,SOD,GSH-Px和CAT酶活性显著升高(P<0.05)。结论番茄红素能有效改善因慢性锰染毒导致的氧化应激损伤及学习记忆能力的减退。OBJECTIVE To investigate the effects of lycopene on learning and memory in rats exposed to manganese chloride. METHODS Adult male rats were ip given manganese chloride 15 mg·kg-1, and lycopene 5, 10 and 15 mg·kg-1 for three months. The escape latency and swimming time were detected by WT-200 water maze at the end of the first, second and the third months. After three months later, the atomic absorption spectrometry method was used to determine the manganese contents in blood, liver and hippocampus. The spectrophotometry was used to determine the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, catalase（CAT） and the contents of maleic dialdehyde （MDA） in liver and hippocampus. RESULTS Compared with normal control and lycopene groups, the escape latency and swimming time in Mn model group significantly increased （P〈0.05）. The manganese contents in blood, liver and hippocampus markedly increased （P〈0.05）. The body mass greatly decreased （P〈0.05）. At the same time, Mn administration induced oxidative stress as evidenced by elevated levels of lipid peroxidation measured as MDA in liver and hippocampus tissues （P〈0.05）. Moreover, the activities of SOD, GSH-Px, and CAT significantly decreased in the liver and hippocampus tissues （P〈0.05）. However, the escape lantency decreased from 50.7±10.8, 46.1±9.2 and （51.4±13.6）s in Mn model group to 42.1±4.0, 27.5±4.6 and （26.0±4.7）s when ig given lycopene 15 mg·kg-1 at the end of the first, second and the third month, respectively （P〈0.05）, the swimming time decreased from 38.7±9.2 and （45.2±14.2）s to 26.2±4.8 and （27.3±8.3）s （P〈0.01）. The manganese contents in blood, liver and hippocampus decreased significantly（P〈0.05）, the MDA contents decreased significantly in liver and hippocampus（P〈0.05）, the activities of SOD, GSH-Px and CAT increased significantly in the liver and hippocampus tissues（P〈0.05）. CONCLUSION Lycopene administration can improve lea

关 键 词：番茄红素 锰染毒 氧化性应激 学习记忆 

分 类 号：R285.5[医药卫生—中药学]